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Microscopy Unit

The Microscopy service of the Institut Pasteur from Montevideo (IP Montevideo) has equipment for performing fluorescence and confocal microscopy. These microscopes are available to all researchers in the public or private sector who wish to view and take pictures of fluorescent or confocal microscopy.

Our service is dedicated to making assistance and image processing as well as providing technical advice. We have high quality equipment that allow to obtain high resolution images of materials of biological and non-biological origins.

MEMBERS

  • Responsibles

    Dr. Flavio Zolessi

    Dr. Federico Lecumberry.

  • Technician

    M.Sc. Marcela Díaz

    Tabaré de los Campos

MAIN EQUIPMENT

Inverted fluorescence microscope

Brand: Olympus

Model: IX81

Camera: ORCA, Hamamatsu

The fluorescence microscope is equipped with dichroic filters that allow observation of a wide range of fluorochromes:

Available fluorescence filters:

  • 1 – U-MNUA2: Excitation 360-370nm / Emission 420-460nm (DAPI)
  • 2 – U-MNIBA3: Excitation 470-495nm / Emission 510-550nm (FITC, GFP)
  • 3 – U-MWIG3: Excitation 530-550nm / Emission 570nm (Rhodamine, TRITC, Cy3, Texas Red)
  • 4- GOLD: Excitation 300-400nm / Emission 515nm (sybr GOLD)

It also has polarizing filters for observations of differential interference contrast (Nomarski technique).

Acquisition Software:

Image pro-plus (SCOPE-PRO)

Available Objectives:

  • 10X NA 0.30
  • 40X LUCP LWD NA 0.6
  • 60X NA 1.25 OIL IMMERSION
  • 100X NA 1.40 OIL IMMERSION

 

Confocal Microscopy

Brand: Leica

Model: DMI6000, TCS-SP5

Available filters:

  • 1 – A: Excitation 340-380nm / Emission 425 (DAPI)
  • 2 – I3: Excitation 450-490nm / Emission 515 (FITC, GFP)
  • 3 – N2.1: Excitation 515-560nm / Emission 590nm (TRITC, Cy3, Texas Red)

Available Objectives:

  • 20X NA 0.70 WATER / GLYCEROL /OIL IMMERSION
  • 63X NA 1.42 OIL IMMERSION

Available Lasers:

  • Argon 488nm: 458/476/488/496/514
  • HeNe 543nm
  • HeNe 594nm
  • HeNe 633nm
  • Diode 405 nm

Software acquisition:

The confocal laser microscope is an essential tool for the study of intracellular localization and colocalization of fluorescently labeled signals. The software LASAF (Leica Application Suite Advanced Fluorescence) allows acquisition and image processing.

Confocal Microscopy II

Brand: Zeiss

Model: Axio Observer Z1, LSM 800

  • Transmitted Light Basis Set with DIC for LSM 800

Available fluorescence filters:

  • 1 – DAPI: Ex 365 nm, Em 445/50
  • 2 – FITC, GFP: Ex 450 – 490, Em 515 – 565
  • 3 –TRITC, RODAMINE: Ex 546/12, Em 575 – 640

Available Objectives:

  • 25X LD LCI Plan-Apochromat 0,8 AN/ WD:0,57/ cover glass 0-0,17.

Imm DIC. cod: 420852-9871-000

  • 63X LCI-plan-Neofluar/1,3 AN/DIC cod: 420882-9970-000
  • Objective i Plan-Apochromat 63x/1.4 Oil DIC M27 with insulation ring for optimized

temperature at the specimen, (WD=0.18mm) incl. Immersol 518 F, oiler 20ml and

Cover glasses, high performance, D=0.17mm

Available Lasers:

  • Diode laser 405nm, 5mW, laser class 3B
  • Diode laser 488nm, 10mW, laser class 3B
  • Diode laser (SHG) 561nm, 10mW, laser

class 3B

  • Diode laser 640nm, 5mW, laser class 3B

Acquisition Software:

ZEN 2.1 system Hardware License Key

Image acquisition and processing under Win 7 x64. User interface configurable, control of the Carl

Zeiss microscope systems and components, extensive

acquisition and analysis. CZI image format.

 

The following modules are included:

– ZEN Module Measurement

– ZEN Module Multi Channel

– ZEN Module Image Analysis

– ZEN Module Time Lapse

– ZEN Module Z Stack

– ZEN Module Extended Focus

– ZEN Module Autofocus

– ZEN Module Colocalisation

– ZEN Module Spectral Unmixing

– ZEN Module Tiles & Positions
services

SERVICES

  • Capture images using confocal microscopy
  • Capture images using epifluorescence microscopy

Services Price List

RESEARCH

EDUCATION-COURSES

Processing and Analysis of Fluorescence Microscopy Images

GRANTS

PUBLICATIONS

CONTACT

microscopia@pasteur.edu.uy