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Cell Biology Unit

MEMBERS

  • Mariela Bollati, PhD (Head)
  • Karen Perelmuter, MSc (Staff TA)
  • Sabina Victoria, MSc (Staff TA)
  • Romina Pagotto, PhD (Postdoctoral fellow)
  • Vanesa Piattoni, PhD (Postdoctoral fellow)
  • Soledad Astrada, MSc (PhD student)
  • Hellen Daghero (Master student)
  • Micaela Sureda (Research internship)

MAIN EQUIPMENT

Cell Culture Facility

This laboratory is equipped with biological safety cabinets, CO2 incubators, centrifuges and inverted microscopes. Established cell line (adherent or suspension), heterotypic and primary cultures are manipulated in this lab. There is a cell bank consisting of more than 20 certified cell lines (ATCC, DSMZ) and other 20 recombinant cell lines. Periodically, cell banks and cell lines are controlled for mycoplasma contamination by PCR.

Flow Cytometry

MoFlo high speed cell sorter (Beckman Coulter)

This instrument has the capability to:

  • Sort a total event rate of over 30,000 per second
  • Analyze a total event rate of over 50,000 per
  • Sort simultaneously up to 4 populations (4-way sorting)
  • Deposite a single cell/well into tissue culture trays

It is equipped with:

CyAn Flow Cytometer (Beckman Coulter)

CyAn ADP can measure the following parameters:

  • Size of a cell: Forward light Scatter or FSC.
  • Granularity of a cell: Side light Scatter or SSC.
  • Fluorescent parameters:
  • Fluorescence resolution: coefficient of variation (CV) is
  • Flow rates: Up to 150 µl/min
  • Processing speed: up to 70,000 events per second.
  • Analysis capability: up to 100,000,000 event data files

Data analysis

The cell biology Unit provides the following flow cytometry data analysis software:

  • SUMMIT 4.3
  • FlowJo 7.6.4
  • FCSExpress 4 Flow research edition

Technical assistance is offered for sample preparation, reagent purchase, experimental design and experimental data analysis.

Trainings and education are available for all the new users.

We are also open to establish collaborations and have an active involvement in your research providing intellectual and practical assistance.

For more information and bookings you can contact us: citometria @ pasteur.edu.uy
services

SERVICES

The CBU in 2007 has installed cell culture facilities and the flow cytometry lab (equipped with a cell sorter MoFlo and an analytical cytometer CyAn ADP, both from Beckman Coulter). By the end of 2014 we have completed the CBU platform with the arrival of a new cell sorter (FACSAria Fusion, BD) which can operate under a Class II Type A2 biosafety cabinet and a small analyser (Accuri C6, BD). Both instruments together complement each other and expand the quality and type of procedures (analytical and preparative) we can perform and offer to local and regional scientific community.

The routine services that we offer are:

  1. Culture of different cell lines, cell banking.
  2. Detection of mycoplasma contamination in cell culture by PCR.
  3. Cell-based assays.
  4. Generation of recombinant stable cell lines.
  5. Flow cytometry analysis: DNA content and cell cycle analysis, fluorescent proteins detection, apoptosis, and multicolor analysis.
  6. Sorting of heterogeneous cell populations into homogeneous populations: sterile sorting, single cell deposition, 4 way sorting.

RESEARCH

The CBU is involved in two main areas of research:

CELL CULTURE TECHNOLOGY

During the last years, our group has generated a variety of reporter cell lines with broader applications (NF- kB, type I IFN, redox biosensors, among others). These stable cell lines are being widely used to search and characterize substances that interfere with the type I IFN signaling pathways (Burgi et al, 2012 and one manuscript in preparation), for the improvement of metabolism / productivity of cells with biotechnological interest (redox biosensors in collaboration with Dr Comini from the Redox Biology of Trypanosomes Lab, IP Montevideo and we have a manuscript in preparation), or for in vitro models of inflammation (NF- kB, Tiscornia et al, 2012; Mastropietro et al, 2015).

ENVIRONMENTAL TOXICOLOGY

A wide variety of anthropogenic substances in the environment, known as endocrine disruptors (EDs), are able to alter the homeostasis of the endocrine system of organisms. In the field of endocrine disruption, the working hypothesis is that the increment of certain reproductive disorders are caused, at least in part, by an increased exposure to substances classified as EDs that are present in the environment. In this context, we focus on the design and development of in vitro and in vivo models for toxicological studies of EDs. For the in vitro approach, we are on the process to obtain a dual reporter cell line, to assess in a single assay the estrogenic or androgenic activity of a putative ED. For the in vivo studies, we proposed and validated the Oct4-GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, as a mammalian model to study the effects of EDs on the development of male germ cells. We introduced the transgenic Oct4/GFP mouse together with flow cytometry as a suitable tool to evaluate changes in male germ cells development and to identify early life exposures to EDCs (manuscript under revision and other in preparation). To accomplished the Project we have the financial support from ANII (2013-2015), one post-doctoral fellow ANII (R. Pagotto), and one initiation fellow ANII (MB. Harreguy). In addition, we are actively collaborating with Dr. H. Rodríguez (Instituto de Salud y Ambiente del Litoral, CONICET-UNL, Santa Fe, Argentina).

COLLABORATIVE PROJECT

Since 2011 we are collaborating with MG. Vallespi, PhD, from the Pharmaceuticals Division, Center for Genetic Engineering and Biotechnology (CIGB), Habana, Cuba in the project entitled “CIGB -552: novel peptide with antitumor properties useful for cancer treatment“. From this collaboration, two articles were published (Fernández Massó et al, 2013, J Amino Acids and Vallespí et al., 2014, J Pept Sc), one manuscript is under revision and there is one in preparation. In addition a student (S Astrada) from the Cell Biology Unit is performing her PhD thesis under the supervision of Bollati M and Vallespi M.

The work in association with Susana Etcheverry, PhD (Cequinor, UNLP, Argentina) have yielded three manuscripts and one is in preparation (Leon et al, 2013, Dalton Trans; Leon et al, 2014; Chem Biol Interact. and Leon et al, 2014, J Biol Inorg Chem).

References

  • Bürgi M, Prieto C, Etcheverrigaray M, Kratje R, Oggero M, Bollati-Fogolín M*. WISH cell line: From the antiviral system to a novel reporter gene assay to test the potency of human IFN-α and IFN-β. J Immunol Methods. 2012, 381(1-2):70-4.
  • Fernández Massó JR, Oliva Argüelles B, Tejeda Y, Astrada S, Garay H, Reyes O, Delgado-Roche L, Bollati-Fogolín M, Vallespí MG. The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells. J Amino Acids. 2013; 251398.
  • Leon IE, Di Virgilio AL, Porro V, Muglia CI, Naso LG, Williams PAM, Bollati-Fogolín M and Etcheverry SB. Antitumor properties of a vanadyl(IV) complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 in a human osteosarcoma model: role of the oxidative stress and apoptosis. Dalton Trans. 2013; 42(33):11868-80.
  • Leon IE, Porro V, Di Virgilio AL, Naso LG, Williams PAM, Bollati-Fogolín M and Etcheverry SB. Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells. J Biol Inorg Chem. 2014, 19 (1), pp. 59-74.
  • León IE, Porro V, Astrada S, Egusquiza MG, Cabello CI, Bollati-Fogolin M, Etcheverry SB. Polyoxometalates as antitumor agents: Bioactivity of a new polyoxometalate with copper on a human osteosarcoma model. Chem Biol Interact. (2014), 222C:87-96.
  • Mastropietro G, Tiscornia I, Perelmuter K, Astrada S, Bollati-Fogolín M*. HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation.Mediators of Inflammation, 2015, Article ID 860534, in press.
  • Tiscornia I., Sánchez-Martins V., Hernández A., Bollati-Fogolín M*. Human monocyte-derived dendritic cells from leukoreduction system chambers after plateletpheresis are functional in an in vitro co-culture assay with intestinal epithelial cells. J Immunol Methods. 2012, 384(1-2):164-70.
  • Vallespí MG, Pimentel G, Cabrales-Rico A, Garza J, Oliva B, Mendoza O, Gomez Y, Basaco T, Sánchez I, Calderón C, Rodriguez JC, Markelova MR, Fichtner I, Astrada S, Bollati-Fogolín M, Garay HE, Reyes O. Antitumor efficacy, pharmacokinetic and biodistribution studies of the anticancer peptide CIGB-552 in mouse models. J Pept Sc (2014), 20(11):850-9.

* Corresponding author

EDUCATION-COURSES

As organizers:

  • Internacional Flow cytometry and cell sorting in biotechnology and biomedicine research course held at the IP Montevideo from April 17th to 28th. Organizing committee: M. Bollati, L. Groeber and JM Garcia. Supporting funds have been obtained from the Reseau International des Instituts Pasteur (RIIP), the International Centre for Genetic Engineering and Biotechnology (ICGEB), United Nations University (UNU-Biolac), and Third World Academy of Sciences (TWAS). Amount Granted: € 35,000 and USD 15,000.
  • 1st Processing and Image Analysis Course Using Icy Software. S. Astrada participated as organizer, December 15-17, Institut Pasteur Montevideo, Uruguay.

As lecturers and instructors:

  • Animal cell culture technology course, PUCV, Valparaiso, Chile. M. Bollati participated as conferencist. November 2014.
  • Biotecnología aplicada a la transferencia y expresión de genes recombinantes en células animales. FBCB, Santa Fe, Argentina. M. Bollati participated as lecturer. September 2014.
  • Curso Básico de Cultivo de Células, PEDECIBA – BIOLOGIA. Montevideo, Uruguay. I. Tiscornia and M. Bollati participated in the theoretical and practical activities. May 2014.

TRAINING OF STUDENTS

In the year 2014 three students defended their thesis and graduated:

  • Milagros Burgi completed her PhD studies at the FBCB, UNL, Santa Fe, Argentina. Supervisors: R. Kratje and M. Bollati
  • Giuliana Mastropietro completed her diploma thesis in Biotechnology, Facultad de Ingeniería, Universidad ORT, Uruguay. Supervisors: I. Tiscornia, K. Perelmuter & M. Bollati
  • Ma Belén Harreguy completed her diploma thesis in Biotechnology, Facultad de Ingeniería, Universidad ORT, Uruguay. Supervisors: R. Pagotto, V. Porro & M. Bollati

Two students are performing their PhD or diploma theis work at the Unit:

  • Soledad Astrada. PhD student at Pro.In.Bio, Uruguay. Supervisorss: M. Bollati and M. Guerra Vallespi
  • Giuliana Mastropietro, diploma work for Biotechnology Engineer degree, Facultad de Ingeniería, Universidad ORT, Uruguay. Supervisors: I. Tiscornia, K. Perelmuter & M. Bollati.

In addition, our Unit has hosted a total of four (4) traineeships for students, postdoctoral fellows and visiting scientist:

  • PhD Natalia Calvo: from Universidad Nacional del Sur, Bahía Blanca, Argentina. February-March 2014 (two months).
  • MSc. Brizaida Oliva: PhD student from Centro de Ingeniería Genética y Biotecnología, Habana, Cuba. April 2014 (one month).
  • Bioq. Clarisa Santamaría: PhD student from Facultad de Bioquímica y Cs. Biológicas, Universidad Nacional del Litoral Santa Fe, Argentina. April 2014 (one month).
  • PhD Maribel Guerra Vallespí: from Centro de Ingeniería Genética y Biotecnología, Habana, Cuba. December 2014 (one month).

RECEIVED TRAINING BY THE CELL BIOLOGY UNIT STAFF

  • All members of the Cell Biology Unit were trained in the requirements of ISO 9001:2008 standard and ISO/IEC 17025:2005 standard. IP Montevideo, Uruguay. February, 2014.
  • K. Perelmuter performed an intership in the Laboratorio de Investigaciones del Sistema Inmune (LISIN), Universidad Nacional de La Plata, Buenos Aires, Argentina. May 2014.
  • R Pagotto accomplished one-month internship at the Facultad de Bioquímica y Cs. Biológicas, Universidad Nacional del Litoral, Santa Fe Argentina. May 2014
  • K. Perelmuter attended to the “Animal Cell Technology course – 4th edition”. Llafranc, Spain. September 2014.
  • K. Perelmuter participated in the “Cells for compound screening course– 1st edition”. Llafranc, Spain. October 2014.
  • S. Victoria completed an internship at the London Research Institute (LRI) in London, UK during three weeks. The objective was to gain experience in flow cytometers and cell sorters. October 2014.

GRANTS

  1. Separador celular de alta velocidad, multiparamétrico y bioseguro para su utilización en biomedicina y biotecnología. PI: M Bollati, ANII (Uruguay), 2014. Granted: USD 480,000.
  2. Toxicología ambiental aplicada: evaluación del riesgo por exposición a estrógenos ambientales antropogénicos en un modelo de ratones transgénicos Oct4-GFP. PI: M Bollati, ANII: FMV (Uruguay), 2013 – 2015 (2 years). Granted: USD 20,000.
  3. Modulación de la respuesta innata epitelial por levaduras probióticas: determinación de los mecanismos genéticos de levaduras involucrados en esta propiedad. PI: M. Bollati (Uruguay), M. Rumbo (Argentina). Cooperación bilateral Argentina–Uruguay (AR-UR/12/03), 2013 – 2015 (2 years). Granted: USD 5,000
  4. Toxicología ambiental aplicada: evaluación del riesgo por exposición a estrógenos ambientales antropogénicos en un modelo murino transgénico Oct4-GFP. PI: M. Crispo, M. Bollati (collaborative partner). Cooperación bilateral Argentina–Uruguay (AR-UR/12/02), 2013 – 2015 (2 years). Granted: USD 5,000
  5. Postdoctoral fellowship CONICET-IPMontevideo, R. Pagotto, 2012-March 2014 (2 years)
  6. Doctoral fellowship ANII- POS_NAC_2012_1_8523, S. Astrada, 2013-2015 (2 years)
  7. Initiation fellowship ANII- INI_X_2012_1_4184, MB. Harreguy, 2013- 2014 (1 year)
  8. Postdoctoral fellowship ANII- PD_NAC_2013_1_10903, R. Pagotto, 2014-2015 (2 years).

PUBLICATIONS

2016

  • García EP, Tiscornia I, Libisch G, Trajtenberg F, Bollati-Fogolín M, Rodríguez E, Noya V, Chiale C, Brossard N, Robello C, Santiñaque F, Folle G, Osinaga E, Freire T. MUC5B silencing reduces chemo-resistance of MCF-7 breast tumor cells and impairs maturation of dendritic cells. Int J Oncol. 2016 Mar 10. doi: 10.3892/ijo.2016.3434.
  • Ubillos L, Freire T, Berriel E, Chiribao ML, Chiale C, Festari MF, Medeiros A, Mazal D, Rondán M, Bollati-Fogolín M, Rabinovich GA, Robello C, Osinaga E. Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers. Int J Cancer. 2016 Apr 1;138(7):1719-31. doi: 10.1002/ijc.29910. Epub 2015 Nov 20.

2015

  • León IE, Cadavid-Vargas JF, Tiscornia I, Porro V, Castelli S, Katkar P, Desideri A, Bollati-Fogolin M, Etcheverry SB. Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model. J Biol Inorg Chem. 2015 Oct;20(7):1175-91. doi: 10.1007/s00775-015-1298-7.
  • Núñez de Villavicencio-Díaz T, Ramos Gómez Y, Oliva Argüelles B, Fernández Masso JR, Rodríguez-Ulloa A, Cruz García Y, Guirola-Cruz O, Perez-Riverol Y, Javier González L, Tiscornia I, Victoria S, Bollati-Fogolín M, Besada Pérez V, Guerra Vallespi M. Data for comparative proteomics analysis of the antitumor effect of CIGB-552 peptide in HT-29 colon adenocarcinoma cells. Data Brief. 2015 Jul 8;4:468-73. doi: 10.1016/j.dib.2015.06.024.
  • Porro V, Pagotto R, Harreguy MB, Ramírez S, Crispo M, Santamaría C, Luque EH, Rodríguez HA, Bollati-Fogolín M. Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry. J Steroid Biochem Mol Biol. 2015 Nov;154:53-61. doi: 10.1016/j.jsbmb.2015.06.006.
  • Salzman V, Porro V, Bollati-Fogolín M, Aguilar PS. Quantitation of yeast cell-cell fusion using multicolor flow cytometry. Cytometry A. 2015 Sep;87(9):843-54. doi: 10.1002/cyto.a.22701.
  • Núñez de Villavicencio-Díaz T, Ramos Gómez Y, Oliva Argüelles B, Fernández Masso JR, Rodríguez-Ulloa A, Cruz García Y, Guirola-Cruz O, Perez-Riverol Y, Javier González L, Tiscornia I, Victoria S, Bollati-Fogolín M, Besada Pérez V, Guerra Vallespi M. Comparative proteomics analysis of the antitumor effect of CIGB-552 peptide in HT-29 colon adenocarcinoma cells. J Proteomics. 2015 Aug 3;126:163-71. doi: 10.1016/j.jprot.2015.05.024.
  • Negrotto S, Mena HA, Ure AE, Jaquenod De Giusti C, Bollati-Fogolín M, Vermeulen EM, Schattner M, Gómez RM. Human Plasmacytoid Dendritic Cells Elicited Different Responses after Infection with Pathogenic and Nonpathogenic Junin Virus Strains. J Virol. 2015 Jul;89(14):7409-13. doi: 10.1128/JVI.01014-15. Epub 2015 Apr 29.
  • Mastropietro G, Tiscornia I, Perelmuter K, Astrada S, Bollati-Fogolín M. HT-29 and Caco-2 reporter cell lines for functional studies of nuclear factor kappa B activation. Mediators Inflamm. 2015;2015:860534. doi: 10.1155/2015/860534.

2014

  • Leon IE, Porro V, Di Virgilio AL, Naso LG, Williams PA, Bollati-Fogolín M, Etcheverry SB. Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells. J Biol Inorg Chem. 2014 Jan;19(1):59-74.
  • Domínguez MF, Koziol U, Porro V, Costábile A, Estrade S, Tort J, Bollati-Fogolin M, Castillo E. A new approach for the characterization of proliferative cells in cestodes. Exp Parasitol. 2014 Mar;138:25-9. doi: 10.1016/j.exppara.2014.01.005
  • Fernández-Calero T, Astrada S, Alberti A, Horjales S, Arnal JF, Rovira C, Bollati-Fogolín M, Flouriot G, Marin M. The transcriptional activities and cellular localization of the human estrogen receptor alpha are affected by the synonymous Ala87 mutation. J Steroid Biochem Mol Biol. 2014 Sep;143:99-104. doi: 10.1016/j.jsbmb.2014.02.016.
  • Beedessee G, Ramanjooloo A, Tiscornia I, Cresteil T, Raghothama S, Arya D, Rao S, Gowd KH, Bollati-Fogolin M, Marie DE. Evaluation of hexane and ethyl acetate extracts of the sponge Jaspis diastra collected from Mauritius Waters on HeLa cells. J Pharm Pharmacol. 2014 Sep;66(9):1317-27. doi: 10.1111/jphp.12256.
  • Vallespí MG, Pimentel G, Cabrales-Rico A, Garza J, Oliva B, Mendoza O, Gomez Y, Basaco T, Sánchez I, Calderón C, Rodriguez JC, Markelova MR, Fichtner I, Astrada S, Bollati-Fogolín M, Garay HE, Reyes O. Antitumor efficacy, pharmacokinetic and biodistribution studies of the anticancer peptide CIGB-552 in mouse models. J Pept Sci. 2014 Jul 20. doi: 10.1002/psc.2676

2013

  • Noya V, Bay S, Festari MF, García E, Rodriguez E, Chiale C, Ganneau C, Baleux F, Astrada S, Bollati–Fogolín M, Osinaga E and Freire T. Mucin-like peptides from Echinococcus granulosus induce antitumor activity. Int J Oncol. 2013 (June 28). DOI: 10.3892/ijo.2013.2000.
  • Chenoll E, Codoñer FM, Silva A, Ibáñez A, Martinez-Blanch JF, Bollati-Fogolín M, Crispo M, Ramírez S, Sanz Y, Ramón D and Genovés S. Genomic Sequence and Pre-Clinical Safety Assessment of Bifidobacterium longum CECT 7347, a Probiotic able to Reduce the Toxicity and Inflammatory Potential of Gliadin-Derived Peptides. J Prob Health 2013, 1: 106. doi: 10.4172/ jph.1000106
  • Leon IE, Di Virgilio AL, Porro V, Muglia CI, Naso LG, Williams PAM, Bollati-Fogolín M and Etcheverry SB. Antitumor properties of a vanadyl(IV) complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 in a human osteosarcoma model: role of the oxidative stress and apoptosis. Dalton Transactions, 2013 (in press).
  • Mazal D, Lo-Man R, Bay S, Pritsch O, Dériaud E, Ganneau C, Medeiros A, Ubillos L, Obal G, Berois N, Bollati-Fogolín M, Leclerc C, Osinaga E. Monoclonal antibodies toward different Tn-amino acid backbones display distinct recognition patterns on human cancer cells. Implications for effective immuno-targeting of cancer. Cancer Immunol Immunother. 2013 Apr 21. doi: 10.1007/s00262-013-1425-7
  • Fernández Massó JR, Oliva Argüelles B, Tejeda Y, Astrada S, Garay H, Reyes O, Delgado-Roche L, Bollati-Fogolín M, Vallespí MG. The Antitumor Peptide CIGB-552 increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells. J Amino Acids. 2013;2013:251398. doi: 10.1155/2013/251398. Epub 2013 Jan 16.

2012

  • Grompone, G; Martorell, P; Llopis, S; González, N; Genovés, S; Mulet, AP; Fernández-Calero, T; Tiscornia, I; Bollati-Fogolín, M; Chambaud, I; Foligné, B; Montserrat, A; Ramón Vidal, D. Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans. PLOS ONE 2012;7(12) doi: 10.1371/journal.pone.0052493
  • Tiscornia, I, Sánchez-Martins, V, Hernández, A, Bollati-Fogolín, M. Human monocyte-derived dendritic cells from leukoreduction system chambers after plateletpheresis are functional in an in vitro co-culture assay with intestinal epithelial cells, Accepted in Journal of Immunological Methods (2012), doi:10.1016/j.jim.2012.07.005
  • Muñoz F, Del Río N, Sóñora C, Tiscornia I, Marco A, Hernández A. Enamel defects associated with coeliac disease: putative role of antibodies against gliadin in pathogenesis. Eur J Oral Sci. 2012 Apr;120(2):104-12. doi: 10.1111/j.1600-0722.2012.00949.x.
  • Bürgi, M.; Prieto, C.; Etcheverrigaray, M.; Kratje, R.; Oggero, M.; Bollati-Fogolín, M. Wish cell line: from the antiviral system to a novel reporter gene assay to test the potency of human IFN-α and IFN-β. Journal of Immunological Methods (2012) Journal of Immunological Methods (2012), 381(1-2):70-4. available online:  http://dx.doi.org/10.1016/j.jim.2012.04.010
  •  Hernández P., Cabrera M., Lavaggi M.L., Celano L., Tiscornia I., Rodrigues da Costa T., Thomson L., Bollati-Fogolín M., Miranda A.L., Moreira-Lima L., Barreiro E., González M. and Cerecetto H. Discovery of new orally effective analgesic and anti-inflammatory hybrid furoxanyl n-acylhydrazone derivatives. Bioorg Med Chem. 2012; 20(6): 2158-71.

2011

  • Bürgi, M.; Prieto, C.; Oggero, M.; Bollati-Fogolín, M.; Etcheverrigaray, M.; Kratje, R. New reporter cell clones to determine the biological activity of human type I interferons. BMC Proceedings 2011, 5(Suppl 8):P4.
  • Gascue, C; Tan, PL; Cardenas-Rodriguez, M.; Libisch, G.; Fernandez-Calero, T.; Liu, YP; Astrada, S.; Robello, C.; Naya, H; Katsanis, N.; Badano, JL. A Direct Role of Bardet-Biedl Syndrome Proteins in Transcriptional Regulation.  Journal of Cell Science.  In Press
  • Pils, M; Bleich, A; Fasnacht, N; Bollati-Fogolín, M; Schippers, A; Rozell, B; Müller, W. Commensal gut flora reduces susceptibility to experimentally induced colitis via T-cell derived Interleukin-10. Inflammatory Bowel Diseases 2011 Oct;17(10):2038-46. PMID:21182023

2010

  • Cabrera M, Lavaggi ML, Croce F, Celano L, Thomson L, Fernández M, Pintos C, Raymondo S, Bollati M, Monge A, López de Ceráin A, Piro OE, Cerecetto H, González M. Identification of chalcones as in vivo liver monofunctional phase II enzymes inducers. Bioorganic & Medicinal Chemistry, v. 18 14, p. 5391-5399, 2010. PMID:20639112
  • Palacios F, Moreno P, Morande P, Abreu C, Correa A, Porro V, Landoni AI, Gabus R, Giordano M, Dighiero G, Pritsch O and Oppezzo P. High expression of AID and active Class Switch Recombination accounts for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease.  Blood 2010 Jun 3;115(22):4488-96 PMID:20233972
  • Guimarães FS, Ferrari LA, Martins SH, Sene RV, Wanderer C, Tiscornia I, Bollati-Fogolín M, Trindade ES, de Freitas Buchi D and Abud APR. In vitro and in vivo anticancer properties of a Calcarea carbonica derivative complex (M8) treatment in a murine melanoma model. BMC Cancer 2010 Mar 25; 10:113 PMID:20338038

2009

  • Madan R, Demircik F, Surianarayanan S, Allen JL, Divanovic S, Trompette A, Yogev N, Gu Y, Khodoun M, Hildeman D, Boespflug N, Fogolin MB, Gröbe L, Greweling M, Finkelman FD, Cardin R, Mohrs M, Müller W, Waisman A, Roers A, Karp CL. Nonredundant roles for B cell-derived IL-10 in immune counter-regulation. J Immunol. 2009, 183(4):2312-20. PMID:19620304
  • Fasnacht N, Greweling MC, Bollati-Fogolín M, Schippers A, Müller W. T-cell-specific deletion of gp130 renders the highly susceptible IL-10-deficient mouse resistant to intestinal nematode infection. Eur J Immunol. 2009, 39(8):2173-83. PMID:19593768
  • Rodríguez-Casuriaga R, Geisinger A, López-Carro B, Porro V, Wettstein R, Folle GA. Ultra-Fast and Optimized Method for the Preparation of Rodent Testicular Cells for Flow Cytometric Analysis. Biol Proced Online. 2009 Mar 6;11:184-95 PMID:19495915

2008

  • Sudarman E; Bollati-Fogolín M; Hafner M; Mueller W; Scheller J; Rose-John S; Eichler J. Synthetic mimetics of the gp130-binding site for viral Interleukin-6 as inhibitors of the vIL-6 – gp130 interaction. Journal of Peptide Research, 2008, v. 71 5, p. 494-500. PMID:18373551
  • Bollati-Fogolín M. and Comini M., Clonagem e expressäo de proteinas heterologas em células animais. In: Leda Castilho, Angela Maria Moraes, Elisabeth Augusto (Eds.). Tecnologia do Cultivo de Céluas Animais: de Biofármacos a Terapia Genica. Ed. 1, São Paulo, Br Roca, 2008, v. 1, p. 40-76.
  • Bollati-Fogolín M. and Comini M. (2007), Cloning and expression of heterologous proteins in animal cells. In: Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Castilho, Moraes, Augusto, Butler (Eds), Published by Taylor & Francis Group. 3:39-73.

CAPÍTULOS DE LIBRO

  • Ceaglio N, Bollati-Fogolín M, Oggero M, Etcheverrigaray M, Kratje R (2014). Chapter 6.2: High cell density cultivation processes. In Animal Cell Biotechnology, Eds Wagner R and Hauser H. De Gruyter. Berlín, Alemania (ISBN 3110278863, 9783110278866), pp. 427 – 454.
  • Bollati-Fogolín, M. and Comini, M. (2008) Clonagem e expressäo de proteinas heterologas em células animais. En: Tecnologia do Cultivo de Céluas Animais: de Biofármacos a Terapia Genica. Castilho, Moraes, Augusto (Eds), Published by Editora Roca Ltda, São Paulo, Brasil, 3: 40-76.
  • Bollati-Fogolín, M. and Comini, M (2007), Cloning and expression of heterologous proteins in animal cells. En: Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Castilho, Moraes, Augusto, Butler (Eds), Published by Taylor & Francis Group. 3:39-73.

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