3rd Annual Workshop on Advanced Microscopy and Biophotonics

>, Novedades>3rd Annual Workshop on Advanced Microscopy and Biophotonics

.

3rd Annual Workshop on Advanced Microscopy and Biophotonics

November 22-26, 2021

Hybrid (on line – on site)

Institut Pasteur de Montevideo | Hospital de Clínicas

x

Organizer: Dr. Leonel Malacrida

International speakers:

  • Dr. Enrico Gratton (UCI-USA)
  • Dr. David Jameson (U. Hawaii-USA)
  • Dr. Richard Day (U. Indiana-USA)
  • Dr. Jay Knutson (NHLBI-NIH-USA)
  • Dr. Srigokul Upadhyayula (U. Berkeley -USA)
  • Dr. Michelle Digman (UCI-USA)
  • Dr. Fernando Stefani (CBION-Argentina)
  • Dr. Luca Lanzanó (U. Catania)
  • Dr. Hernán Grecco (UBA-Argentina)
  • Dr. Giuseppe Vicidomini (ITT-Italy)
  • Dr. Susana Sánchez (U. Concepción-Chile)
  • Dr. Valeria Levi (UBA-Argentina)
  • Dr. Per Niklas Hedde (UCI-USA)
  • Dr. Suman Ranjit (Georgetown U-USA)
  • Dr. Diego Presman (UBA-Argentina)
  • Dr. Alexander Dvornikov (UCI-USA)
  • Dr. Laura Estrada (UBA-Argentina)
  • Dr. Belén Torrado (UCI-USA)
  • Dr. Lorenzo Scipioni (UCI-USA)
  • Dr. Giulia Tedeschi (UCI-USA)
  • Dr. Alexander Vallmitjana (UCI-USA)
  • Dr. Francesco Palomba (UCI-USA)

 

Local speakers:

  • Dr. Paola Lepanto (IPMon-FCien-Uruguay)
  • Dr. Federico Lecumberry (FIng-IPMon-UdelaR)

x

Topics:

  • Fluorescence Correlation Spectroscopy (FCS)
  • Photon counting Histogram (PCH)
  • Raster Image Correlation Spectroscopy (RICS)
  • Number and Brightness (N&B)
  • Pair Correlation Function (pCF)
  • Image Mean Square Displacement (iMSD)
  • Fluorescence Lifetime Imaging (FLIM)
  • Förster Resonance Energy Transfer (FRET)
  • Phasor plots (lifetime/spectral)
  • Hyperspectral Imaging and Spectral Phasors
  • 3D Particle Tracking (PT)
  • Deep tissue imaging, DIVER microscopy
  • Single plane illumination microscopy (SPIM)
  • Super-resolution microscopy
  • Advanced imaging processing and AI

x

Course target and goals:

The workshop in Advanced Microscopy and Biophotonics is dedicated to disseminate cutting-edge technology and advanced methods in fluorescence microscopy and biophotonics. The target audience includes postgraduate students, postdocs, and academics from all Americas with a background in the fundamentals of fluorescence microscopy, seeking to learn about recent advances in the combination of spectroscopy with fluorescence microscopy.

In the course, we will cover the fundamentals of advanced instrumentation and methods, such as FCS, FLIM, Hyperspectral imaging, Super-resolution, Non-linear microscopy, Deep tissue imaging, light-sheet microscopy, among others. Due to the global COVID outbreak, this year the workshop will be held as a hybrid version.

The course is organized with lectures of fundamentals (40+5 min), application talks (20+5 min), and an optional advanced section for a virtual practical section (two section 3hs/each) and discussions. This last section is restricted to 20 students and will be given on the last day.

Regional participants will be able to apply for a limited number of fellowships (5). The lectures will be given in English. Due to the pandemic situation, the physical participants will need to demonstrate proof of vaccination and negative test of PCR before the course beginning if coming from outside the country.

.

Inscriptions deadlines:
October 3, 2021 (travel fellowships/local participants)
October 31, 2021 (online participants)

Registration: https://bit.ly/3gCv2fz

On-line transmission:
Institut Pasteur Montevideo YouTube channel

For further information, please contact cursos@pasteur.edu.uy 

Workshop agenda:

(Click here to download the preliminary programme)

Monday November 22

09:00 – 09:15 – Opening and course introduction. Leonel Malacrida
09:15 – 10:15 – Lecture 1. David Jameson. Introduction to fluorescence fundamentals and methods.
10:15 – 11:00 – Lecture 2. Leonel Malacrida: Advanced fluorescence instrumentation and microscopy.

Virtual coffee break and meet with the instructors

11:15 – 12:15 – Lecture 3. Enrico Gratton: Introduction to fluorescence correlation spectroscopy (FCS) and the photon counting histogram (PCH).

Virtual lunch break and meet with the instructors

13:30 – 14:15 – Lecture 4. Per Niklas Hedde: Raster image correlation spectroscopy (RICS) and in vivo applications.
14:15 – 15:00 Lecture 5. Per Niklas Hedde: Number & Brightness (N&B) and its applications for protein oligomerization studies in vivo.

Virtual coffee break and meet with the instructors

15:15 – 16:00 – Lecture 6. Enrico Gratton: The pair-correlation function and its applications for deciphering the occurrence of barriers and obstacles inside the cell.
16:00 – 16:25 – Application talk #1. Valeria Levi: FCS applications for protein dynamics within the nucleus.
16:25 – 16:50 – Application talk #3. Diego Presman: Glucocorticoid receptors oligomeric state at the cell nucleus using N&B.

Virtual coffee break and meet with the instructors

Tuesday November 23

09:00 – 09:45 Lecture 7. David Jameson: Introduction to lifetime measurements and the phasor plots.
09:45 – 10:30 – Lecture 8. Enrico Gratton: The phasor plots for FLIM and FRET.

Virtual coffee break and meet with the instructors

10:45 – 11:30 – Lecture 9. Richard Day: Fluorescent Proteins: Monitoring Dynamic Protein Interactions in the Living Cell Nucleus by FLIM.
11:30 – 12:15 – Lecture 10. Leonel Malacrida: The spectral phasor plot and its applications to hyperspectral imaging.

Virtual lunch break and meet with the instructors

13:30 – 14:15 – Lecture 11. Alex Vallmitjana: Multi-component analysis on FLIM data with the phasor approach.

14:15 – 15:00 – Lecture 12. Suman Ranjit: FLIM-Phasor for cell metabolism phenotyping.

Virtual coffee break and meet with the instructors

15:15 – 16:00 – Lecture 13. Susana Sánchez: Phasor plots for LAURDAN fluorescence and membrane dynamics studies.
16:00 – 16:25– Application talk #4. Jay Knutson. FLIM and new oxygen sensor for in vivo imaging.
16:25 – 16:50 – Application talk #5. Leonel Malacrida: In vivo macromolecular crowding studies in zebrafish lens by ACDAN and spectral imaging.

Virtual coffee break and meet with the instructors

Wednesday November 24

09:00 – 09:45 – Lecture 14. Luca Lanzanó: Fundamentals of Super-resolution microscopy and its combination with spectroscopy.
09:45 – 10:30 – Lecture 15. Fernando Stefani. Far-field fluorescence nanoscopy with sub-10 nm resolution.

Virtual coffee break and meet with the instructors

10:45 – 11:30 – Lecture 16. Giuseppe Vicidomini: Multi-Parameter Live-Cell Observation of Biomolecular Processes with Single-Photon Detector Array.
11:30 – 12:15 – Lecture 17. Leonel Malacrida: The single plane illumination microscopy and its applications to fluorescence spectroscopy.

Virtual lunch break and meet with the instructors

13:30 – 14:15 – Lecture 18. Srigokul Upadhyayula. MOSAIC: Multimodal Optical Scope with Adaptive Imaging Correction for high-spatiotemporal in vivo super-resolution studies.
14:15 – 15:00 – Lecture 19. Alexander Dvornikov: The DIVER microscope for deep tissue imaging.

Virtual coffee break and meet with the instructors

15:15 – 16:00 – Lecture 20. Enrico Gratton: 3D-Single particle tracking and its applications.
16:00 – 16:25 – Application talk #6. Lorenzo Scipioni: Applications of sFLIM-Phasor for in vivo dynamics studies.
16:25 – 16:50– Application talk #7. Belen Torrado: Spectral imaging under highly scattering condition by up and down filters.

Virtual coffee break and meet with the instructors

Thursday November 25

10:00 – 10:25– Application talk #10. Laura Estrada: Deciphering the Dengue virus shuttling in the cell
byline and 2D-pCF analysis.
10:25 – 10:50– Application talk #11. Paola Lepanto: Application of spectral phasor plot for lipidic signatures studies in vivo.
10:50 – 11:15 – Application talk #12. Federico Lecumberry: Machine Learning applied to imaging processing and quantification.
11:15 – 11:40– Application talk #13. David Jameson: TBC
11:40 – 12:05 – Application talk #14. Hernan Grecco: CASPAM: A Triple-Modality Biosensor for Multiplexed Imaging of Caspase Network Activity.

Virtual lunch break and meet with the instructors

13:30 – 13:55 – Application talk #15. Giulia Tedeschi: Fluorescence Fluctuation Spectroscopy enables quantification of potassium channel subunit dynamics and stoichiometry.
13:55 – 14:20 – Application talk #16. Francesco Palomba: Dimethylaminonaphtalen (DAN) probes and liquid phase separation.
14:20 – 14:45 – Application talk#17. Michelle Digman: Multiplexed bioluminiscence by sin/cos filters and phasor approach.
14:45 – 15:10 – Application talk#18. TBC

Virtual coffee break and meet with the instructors

15:40 – 16:40 – Lecture 21: Workshop close and special lecture. David Jameson: A nano-history of fluorescence.
16:40 – 17:30 –Workshop closing, group picture and virtual coffee break with instructors

Friday November 26
09:00 -12:00 – Hybrid practical sections (two parallel), only 20 participant will attend to this semi-virtual section, after course ends videos will be available).
• Two topics (total 10 students by subgroup)
• Spectral phasor for hyperspectral imaging (Leonel Malacrida, Andres Kamaid and Marcela Diaz)
• FLIM-Phasor-Autofluorescence and metabolism (Belén Torrado and Leonel Malacrida)

Virtual lunch break and meet with the instructors

13:30 – 15:30 – Computer room work and presentation preparation
15:30 – 16:30 – Practical section presentation and discussions
16:30-17:00 – Workshop closing and group picture

.

.