Programmes Archives - Institut Pasteur https://pasteur.uy/category/programas/ Tue, 19 Nov 2024 14:39:25 +0000 en-US hourly 1 https://wordpress.org/?v=6.7.1 https://pasteur.uy/wp-content/uploads/2022/04/cropped-favicon-ip-32x32.png Programmes Archives - Institut Pasteur https://pasteur.uy/category/programas/ 32 32 Medical Genomics https://pasteur.uy/en/programmes/human-health/medical-genomics/ Fri, 11 Mar 2022 15:58:14 +0000 https://pasteur.uy/uncategorized/medical-genomics/

Medical Genomics

The objective of the Medical Genomics program is to combine and enhance the capacities of different laboratories and units of the institute in an area of growing scientific interest that links genetics tools and knowledge with its clinical application.

Since the DNA structure was decoded, andgene functions and mutation were linked to human diseases development, genetic research applied to medicine has gained ground in public health policies.
The advances in sequencing techniques has promoted the development of medical genomics and has led to the inclusion of new knowledge in clinical practice.

In this line, at the IP Montevideo, there are several groups that are using genomic techniques and are involved with this programme. These groups are the IP Montevideo’s Bioinformatics Unit, Host-Pathogen Interactions Laboratory and Functional Genomics Laboratory.

]]> Cancer https://pasteur.uy/en/programmes/human-health/cancer/ Fri, 11 Mar 2022 15:53:05 +0000 https://pasteur.uy/uncategorized/cancer/

Cancer

This program is a joint collaboration of the Chronic Lymphoid Leukemia Laboratory, the Glycobiology and Tumor Immunology Laboratory, the Immunoregulation and Inflammation Laboratory, and the Biotechnology in Laboratory Animals Unit (UBAL).

]]> Animal Health https://pasteur.uy/en/programmes/animal-health/ Fri, 11 Mar 2022 15:41:37 +0000 https://pasteur.uy/uncategorized/animal-health/

Animal Health

The Animal Health program involves the joint work of the Immunovirology Laboratory, the Molecular and Structural Microbiology Laboratory, the Host-Pathogen Interaction Laboratory and the Bioinformatics Unit.

]]> Human Health https://pasteur.uy/en/programmes/human-health/ Fri, 11 Mar 2022 15:29:44 +0000 https://pasteur.uy/uncategorized/human-health/

Human Health

The Human Health program involves the work of different laboratories and units of the institute that run research initiatives focused on three different areas.

]]> INDICyO https://pasteur.uy/en/programmes/human-health/indicyo/ Fri, 11 Mar 2022 15:22:20 +0000 https://pasteur.uy/uncategorized/indicyo/

INDICyO

Created in 2016, the Research in Diabetes, Inflammation, Cardiovascular Diseases and Obesity program (INDICyO) is a multidisciplinary initiative that brings together four laboratories of the institute to collaborate in the study of these chronic diseases.

These pathologies of metabolism —defined by the World Health Organization (WHO) as the XXI century epidemic— are associated with aging, poor eating habits and sedentary lifestyle. Its incidence has increased alarmingly in the last 60 years and is currently the leading cause of death in Uruguay and worldwide. Although these diseases are preventable —especially through the promotion of better life habits— it is important to develop new strategies that allow improving their prognosis.

At a biological level, since these diseases are characterized by a chronic inflammatory response in their molecular bases (like arterial hypertension and atherosclerosis, among others), it is key to understand the mechanism that could lead to the development of alternative therapies, whether at the pharmacological or genetic. It is also important to collaborate in the design of public health strategies that improve the incidence and prognosis of these diseases in Uruguay.

The program aims to place the Institut Pasteur de Montevideo as themain biomedical research center for these diseases in Uruguay, bringing together in-house researchers and experts from other national and international academic institutions that also work on these issues. In addition, within the International Network of Pasteur Institutes (RIIP), IP Montevideo also seeks to strengthen its position to become a reference in this area.

Members
Projects
Main equipment
Courses
Main publications

Members

Carlos Escande, PhD

Carlos Escande, PhD

Head

Projects

TMEM176b: a new regulator of inflammation and insulin resistance during obesity. Co-PI: Marcelo Hill (LIRI). International funds and own resources.

Role of FSTL1 in the regulation of adipogenesis and adipose tissue function during obesity. Co-PI: José Badano (GMH). Funded by own resources. International grant (ADA, USA).

Evaluation of new anti-inflammatory compounds for the treatment of metabolic diseases. Co-PI: Carlos Batthyány (UBYPA). Funded by a private international company.

Since its creation, the program has achieved the following milestones:

  • Four doctoral students and two master students share advisors among INDICYO members and develop collaborative research projects.
  • Two research projects with international financing and three with funding from the institute.
  • Weekly seminars with PI and students for documents and results discussions.
  • Two patents and one document presented.

Main equipment

Facilities

  • Bioterium: SPF (specific pathogen free) and Conventional
  • Microinjection Laboratory
  • Assisted Reproduction Techniques Laboratory (ART)
  • Molecular Biology Laboratory

 

Services

  • Generation of transgenic animals using: CRISPR / Cas9 technology, pronuclear microinjection of DNA fragments, gene-targeting in embryonic stem cells, Sleeping Beauty Transposons technology.
  • Cryopreservation of embryos and sperm.
  • Rederivation of murine lines.
  • In vitro fertilization of murine lines.
  • Crosses and maintenance under SPF and conventional conditions of various strains mice (C57BL / 6J, BALB / cJ, DBA / 2J, Nude, several hybrids and about 30 different transgenic lines). Approximate production (currently): 2.000 mice per month.
  • Immunomodulatory capacity assays of probiotic bacteria.
  • Biological activity assays of recombinant erythropoietin. Our Bioterio is certified by the Ministry of Public Health.
  • Toxicity tests for biotechnological products (EPO, Filgen, Interferon).
  • Production of polyclonal antibodies in mice.

 

Training

We offer training in:

  • Techniques for the production of transgenic animals and assisted reproduction.
  • Management of BSL2 pathogens in experimental procedures.

For further information, please contact ubal@pasteur.edu.uy

Courses

  • CARD-IP Montevideo Practical course: Cryopreservation of embryos and murine sperm. Sept. 10-13, 2018. Institut Pasteur Montevideo. Funded by: UNI-Biolac and IP Montevideo. Supported by: CARD and Kumamoto University.
  • “Alternative Methods to the Use of Experimental Animals: In vitro evaluation of irritation (TG 439) and skin sensitization (TG 442E)”. May 21-24, 2018. Institut Pasteur de Montevideo. Funded by: Premasul and L’Oréal. Supported by: Protemca, Aucytal, CNEA.
  • “Theoretical-Practical Course of Laboratory Animals”. March 13-21, 2017. 19 national and one international speakers, and 21 national students. Supported by FOCEM and PEDECIBA.
  • “Integrating IP Montevideo technologies”. Internal course of the IP Montevideo. October 2017.
  • “TRANSGENIC TECHNOLOGIES in MODELING HUMAN DISEASES: Principles, Associated technologies, Animal Management and Ethics”. – June 2017, Athens, Greece. Co-organizers.
  • “Theoretical-practical course of laboratory animals”, authorized for B-technical accreditation by the CNEA. April 4-12, 2016.
  • International course and symposium “ADVANCES IN THE GENERATION OF GENETICALLY MODIFIED (GM) ANIMAL MODELS – International Mini Symposium: TRANSGENIC TECHNOLOGIES, THE LATEST TRENDS”. September 2015.
  • “Management, techniques of substance administration and sampling in mice”. Internal course for 20 researchers who use mice in the IP Montevideo Animal Facility. 2012-2015. 2012-2015.
  • International course “Genetics of laboratory rodents”. December 2008 and 2011. Co-organizer with the Functional Genetics Unit of the Institut Pasteur de Paris.

Main publications

vacio
2018
  • 1. Meikle MN, Schlapp G., Menchaca A., Crispo M. Minimum volume spatula MVD vitrification method improves embryo survival compared to traditional slow freezing, both for in vivo and in vitro produced mice embryos. Cryobiology.
  • 2. Barrera N, Dos Santos Neto PC, Cuadro F, Bosolasco D, Mulet AP, Crispo M, Menchaca A. Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes. PLoS One 13(6). (hyperlink en el t{itulo del art{iculo_ https://www.ncbi.nlm.nih.gov/pubmed/29912910)
2017
  • 1. dos Santos-Neto P.C., Cuadro F., Barrera N., Crispo M., Menchaca A. 2017. Embryo survival and birth rate after minimum volume vitrification or slow freezing of in vivo and in vitro produced ovine embryos. Cryobiology. 78(8): 14.
  • 2. Menchaca A., Schlapp G., Meikle M.N., Crispo M. 2017. Transgenesis and Gene Edition in Mammals. Reference Module in Life Sciences. Elsevier. V.1: 1-9 pp (Book chapter).
  • 3. Menchaga A., AP Mulet, P. Dos Santos Neto, M. Crispo 2017. Transgenesis and Gene Edition in Small Ruminants. Revista Brasileira de Reprodução Animal, v.: 41 1, 2017
  • 4. Mulet AP, Perelmuter K, Bollati-Fogolin M, Crispo M, Grompone G. 2017. Forkhead Box Protein O1 is Linked to Anti-Inflammatory Probiotic Bacteria Acting through Nuclear Factor-κB Pathway. J Microb Biochem Technol Volume 9(3):074-081
2016
  • 1. A. MENCHACA; I. ANEGóN; B. WHITELAW; H. BALDASSARRE; M. CRISPO- New insights and current tools for genetically engineered (GE) sheep and goats. Theriogenology, p.: 1 – 10, 2016.
  • 2. • A. MENCHACA; N. BARRERA; P. DOS SANTOS-NETO; F. CUADRO; M. CRISPO. Advances and limitations of in vitro embryo production in sheep and goats. Animal Reproduction, v.: 13 3, p.: 273 – 278, 2016.
2015
  • 3. Crispo M, Mulet AP, Tesson L, Barrera N, Cuadro F, dos Santos-Neto PC, Nguyen TH, Crénéguy A, Brusselle L, Anegón I, Menchaca A. Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes. PLoS One. 2015 Aug 25;10(8):e0136690. doi: 10.1371/journal.pone.0136690
  • 4. Schlapp G, Goyeneche L, Fernández G, Menchaca A, Crispo M. Administration of the nonsteroidal anti-inflammatory drug tolfenamic acid at embryo transfer improves maintenance of pregnancy and embryo survival in recipient mice. J Assist Reprod Genet. 2015 Feb;32(2):271-5. doi: 10.1007/s10815-014-0378-x. Epub 2015 Jan 6.
  • 5. P. DOS SANTOS; M. VILARIÑO; N. BARRERA; F. CUADRO; M. CRISPO; A. MENCHACA. Cryotolerance of Day 2 or Day 6 in vitro produced ovine embryos after vitrification by Cryotop or Spatula methods. Cryobiology, v.: 70, p.: 17 – 22, 2015.
2014
  • 6. Crispo M, Vilariño M, dos Santos-Neto PC, Núñez-Olivera R, Cuadro F, Barrera N, Mulet AP, Nguyen TH, Anegón I, Menchaca A. Embryo development, fetal growth and postnatal phenotype of eGFP lambs generated by lentiviral transgenesis. Transgenic Res. 2015 Feb;24(1):31-41. doi: 10.1007/s11248-014-9816-x. Epub 2014 Jul 22.
2013
  • 7. Crispo M, Van Maele L, Tabareau J, Cayet D, Errea A, Ferreira AM, Rumbo M, Sirard JC. Transgenic mouse model harboring the transcriptional fusion ccl20- luciferase as a novel reporter of pro-inflammatory response. PLoS One. 2013 Nov 12;8(11):e78447. doi: 10.1371/journal.pone.0078447.
  • 8. M. CRISPO; SCHLAPP G; CÁRDENAS M; D. GONZÁLEZ-MACIEL; M RUMBO. Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line. EJB Electronic Journal of Biotechnology, v.: 16 6, 2013.
2010
  • 9. A. MENCHACA; M. VILARIÑO; M. CRISPO; DE CASTRO T; E RUBIANES. New approaches to superovulation and embryo transfer in small ruminants. Reproduction Fertility and Development, v.: 22 1, p.: 113 – 118, 2010.
2007
  • 10. A. MENCHACA; M VILARIÑO; M. CRISPO; A PINCZAK; E RUBIANES. Day 0 Protocol: superstimulatory treatment initiated in absence of a dominant follicle improves ovarian response and embryo yield in goats. Theriogenology, v.: 68, p.: 1111 – 1117, 2007.

]]> ProTeMCA https://pasteur.uy/en/programmes/protemca/ Fri, 11 Mar 2022 15:06:53 +0000 https://pasteur.uy/uncategorized/protemca/

ProTeMCA

The Molecular, Cellular and Animal Technology Program (ProTeMCA) has as mission to integrate the knowledge and technological capabilities of different groups of the Institut Pasteur of Montevideo to promote the development of research and innovative technology in the area of genetic engineering, bioassays and models based in proteins, cells or animals.

Stressing the link of scientific knowledge with its application, through dynamic interaction with academia and industry, the program seeks to contribute to solve problems in biotechnology using new tools, products, processes or services. It also collaborates with research groups or private institutions to develop molecular technologies that help the improvement of (bio) drugs, cells or animals.

Within the IP Montevideo, the program involves the joint work of the Redox Biology of Trypanosomatids Laboratory, theBiomolecular Simulations Laboratory, the Biotechnology in Laboratory Animals Unit and the Cell Biology Unit.

Members
Research lines
Courses
Projects
Main publications

Members

Cecilia Abreu, PhD

Cecilia Abreu, PhD

Associate researcher

Claudia Ortega, PhD

Claudia Ortega, PhD

Senior associate technician

Lucía Bassetti, BSc

Lucía Bassetti, BSc

Master's degree student

Florencia Fadel, BSc

Florencia Fadel, BSc

Master's degree student

Gabriela Paradeda, BSc

Gabriela Paradeda, BSc

Master's degree student

FOUNDING MEMBERS

Mariela Bollati, PhD

Mariela Bollati, PhD

Cell Biology Unit

Marcelo Comini, PhD

Marcelo Comini, PhD

Redox Biology of Trypanosomatids Laboratory

DVM Martina Crispo, PhD

DVM Martina Crispo, PhD

Biotechnology in Laboratory Animals Unit

Sergio Pantano, PhD

Sergio Pantano, PhD

Biomolecular Simulations Laboratory

Research lines

Design and refinement of products of biotechnological interest.

Development of new high performance cellular assays.

Cell or animal models for toxicological studies and diseases.

Courses

  • “XTREME- Bruker in vivo imaging technology”. February 2017, Montevideo.
  • “ICGEB Course: Cell and animal models for drug discovery”. October 2017, Montevideo.
  • “Alternatives methods to animal research”. May 2018, Montevideo.

Projects

2019-2021 – Generation of extracts rich in polyphenols from grape pomace. Alliance for Innovation- Technological Development Modality. ANII ALI_2_2018_1_149574. Collaborators: Mariela Bollati and Marcelo Comini.

2019- 2021– New molecular tools for the reduction of production costs of bioactive molecules (FMV_3_2018 _l_148443, ANII). Head: Cecilia Abreu

2016-2019 – Design and production of new variants of the follicle stimulating hormone (FSH) for its use in species of productive interest – National Agency for Research and Innovation, ANII (ALI_1_2015_1_5084). Principal Investigator: Mariela Bollati-Fogolin.

2015-2018 – Design of biosensors for simultaneous monitoring of redox signaling and cAMP: from the computer to the cell and back to the computer – National Agency for Research and Innovation, Project FMV_1_2014_1_104000. Main investigator: Sergio Pantano.

Main publications

vacio
2021
  • Arévalo A.P., Pagotto R., Pórfido J. L., Daghero H., Segovia M., Yamasaki K., Varela, Hill M., Verdes J. M., Duhalde Vega M., Bollati-Fogolín M. & Crispo M. (2021). Ivermectin reduces in vivo coronavirus infection in a mouse experimental model. Sci Rep 11, 7132. https://doi.org/10.1038/s41598-021-86679-0
  • Klein F, Sardi F, Machado MR, Ortega C, Comini MA, Pantano S. CUTie2: The Attack of the Cyclic Nucleotide Sensor Clones. Front Mol Biosci. 2021 Mar 11;8:629773. doi: 10.3389/fmolb.2021.629773. PMID: 33778003; PMCID: PMC7991088.
2020
  • Parakh S, Shadfar S, Perri ER, Ragagnin AMG, Piattoni CV, Fogolín MB, Yuan KC, Shahheydari H, Don EK, Thomas CJ, Hong Y, Comini MA, Laird AS, Spencer DM, Atkin JD. (2020). The Redox Activity of Protein Disulfide Isomerase Inhibits ALS Phenotypes in Cellular and Zebrafish Models. iScience; 23(5):101097. doi: 10.1016/j.isci.2020.101097.
  • Medeiros A, Benítez D, Korn RS, Ferreira VC, Barrera E, Carrión F, Pritsch O, Pantano S, Kunick C, de Oliveira CI, Orban OCF, Comini MA. Mechanistic and biological characterisation of novel N5-substituted paullones targeting the biosynthesis of trypanothione in Leishmania. J Enzyme Inhib Med Chem. 2020 Dec;35(1):1345-1358. doi: 10.1080/14756366.2020.1780227. PMID: 32588679; PMCID: PMC7717452
2019
  • Piattoni CV, Sardi F, Klein F, Pantano S, Bollati-Fogolin M, Comini M. New red-shifted fluorescent biosensor for monitoring intracellular redox changes. Free Radic Biol Med. 2019 Feb 5;134:545-554

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