{"id":24850009,"date":"2025-01-24T11:43:54","date_gmt":"2025-01-24T14:43:54","guid":{"rendered":"https:\/\/pasteur.uy\/cell-biology-unit\/"},"modified":"2025-01-24T11:43:59","modified_gmt":"2025-01-24T14:43:59","slug":"cell-biology-unit","status":"publish","type":"page","link":"https:\/\/pasteur.uy\/en\/cell-biology-unit\/","title":{"rendered":"Cell Biology Unit"},"content":{"rendered":"

[et_pb_section fb_built=”1″ fullwidth=”on” disabled_on=”on|on|off” _builder_version=”4.14.6″ _module_preset=”default” height=”200px” max_height=”200px” hover_enabled=”0″ global_colors_info=”{}” background_enable_video_mp4=”off” title_text=”biologia-celular-thumb” background_image=”https:\/\/pasteur.uy\/wp-content\/uploads\/2023\/12\/biologia-celular-thumb.png” sticky_enabled=”0″][\/et_pb_section][et_pb_section fb_built=”1″ custom_padding_last_edited=”on|phone” admin_label=”Advanced Tabs Demo 8″ _builder_version=”4.14.6″ background_color=”#FFFFFF” background_enable_image=”off” custom_padding=”100px||120px|||” custom_padding_tablet=”0px||4%||false|false” custom_padding_phone=”0px||60px||false|false” collapsed=”on” global_colors_info=”{}” transform_styles__hover_enabled=”off|desktop” transform_scale__hover_enabled=”off|desktop” transform_translate__hover_enabled=”off|desktop” transform_rotate__hover_enabled=”off|desktop” transform_skew__hover_enabled=”off|desktop” transform_origin__hover_enabled=”off|desktop”][et_pb_row custom_padding_last_edited=”on|tablet” _builder_version=”4.14.6″ _module_preset=”default” max_width=”90%” custom_padding=”0px||0px||false|false” custom_padding_tablet=”6%||||false|false” custom_padding_phone=”6%||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.14.6″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.14.6″ _module_preset=”default” header_font=”|700|||||||” header_text_color=”#005e9c” header_font_size=”2.5em” global_colors_info=”{}”]<\/p>\n

Services of the Cell Biology Unit<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.14.6″ _module_preset=”default” max_width=”90%” custom_padding=”2%||2%||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dipi_advanced_tabs tab_animation=”fadeInRightShort” dipi_animation_duration=”400″ use_tabs_fullwidth_tablet=”off” tabs_min_width_tablet=”100%” tabs_max_width_tablet=”100%” use_tabs_fullwidth_phone=”off” tabs_min_width_phone=”100%” tabs_placement=”row” tabs_container_width=”27%” tabs_placement_tablet=”column” tabs_align=”center” tabs_container_padding=”40px|0px|40px|0px|false|false” tabs_container_margin=”10px|0px|10px|0px|false|false” tabs_item_padding=”15px|10px|15px|20px|false|false” tabs_item_margin=”0px|0px|0px|2px|false|false” tabs_active_item_padding=”15px|10px|15px|25px|false|false” tabs_active_item_margin=”0px|0px|0px|2px|false|false” use_scroll_to_content=”off” content_padding=”50px|50px|50px|50px|false|false” content_margin=”10px|10px|10px|0px|false|false” tabs_icon_margin=”0px|10px|0px|0px|false|false” tabs_icon_margin_active=”0px|10px|0px|0px|false|false” content_bg_color=”RGBA(255,255,255,0)” tabs_bg_color=”rgba(202,205,207,0)” tabs_item_bg_color=”RGBA(255,255,255,0)” tabs_item_active_bg_color=”rgba(202,205,207,0.1)” tabs_container_padding_tablet=”0px|0px|0px|0px|false|false” tabs_container_padding_phone=”0px|0px|0px|0px|false|false” tabs_container_padding_last_edited=”on|tablet” use_scroll_to_content_tablet=”off” use_scroll_to_content_phone=”on” use_scroll_to_content_last_edited=”on|phone” content_padding_tablet=”50px||50px||false|false” content_padding_phone=”20px|20px|20px|20px|false|false” content_padding_last_edited=”on|phone” _builder_version=”4.14.6″ _module_preset=”default” title_font=”Titillium Web||||||||” title_text_color=”#000000″ title_font_size=”1.2em” title_active_font=”|600|||||||” title_active_text_color=”#e39f15″ title_active_font_size=”1.2em” subtitle_font=”Titillium Web||||||||” subtitle_text_color=”#000000″ body_font=”Titillium Web||||||||” body_text_color=”#000000″ body_font_size=”1.2em” header_3_font=”Montserrat||||||||” header_4_font=”|600|||||||” header_4_text_color=”#005e9c” header_4_font_size=”1.15em” custom_button=”on” button_bg_color=”#000000″ button_font=”Titillium Web|600|||||||” button_icon=”||divi||400″ button_icon_color=”#FFFFFF” hover_enabled=”0″ body_font_size_tablet=”” body_font_size_phone=”15px” body_font_size_last_edited=”on|phone” border_width_right_tabs_wrapper=”2px” border_color_right_tabs_wrapper=”rgba(21,2,42,0.09)” border_width_right_tabs_item=”2px” border_color_right_tabs_item=”rgba(68,0,153,0)” border_width_right_tabs_item_active=”2px” border_color_right_tabs_item_active=”#005e9c” global_colors_info=”{}” tabs_item_padding__hover_enabled=”on|hover” tabs_item_padding__hover=”|||25px|false|false” sticky_enabled=”0″][dipi_advanced_tabs_item title=”Cell culture technology” admin_label=”Tecnolog\u00eda de cultivos celulares” img_container_max_width=”38%” content_image_align=”start” use_button=”on” at_button_text=”Contract” at_button_url=”mailto: vgorrostorrazo@pasteur.edu.uy, ubc@pasteur.edu.uy” at_button_url_new_window=”1″ tab_media=”none” font_icon=”\uf248||fa||400″ font_icon_active=”\uf248||fa||400″ _builder_version=”4.14.6″ _module_preset=”default” custom_button=”on” button_text_size=”14px” button_text_color=”#FFFFFF” button_bg_color=”#000000″ button_border_width=”0px” button_border_color=”#e39f15″ button_border_radius=”0px” button_font=”|600|||||||” button_use_icon=”on” button_icon=”||divi||400″ button_on_hover=”off” button_alignment=”left” button_custom_margin=”30px||||false|false” button_custom_padding=”10px|35px|10px|20px|false|false” hover_enabled=”0″ global_colors_info=”{}” button_bg_color__hover_enabled=”on|hover” button_border_color__hover_enabled=”on|desktop” button_border_color__hover=”#440099″ button_bg_color__hover=”rgba(68,0,153,0.8)” button_bg_enable_color__hover=”on” sticky_enabled=”0″]<\/p>\n

Culture, amplification and storage of different cell lines.<\/h4>\n

<\/strong><\/p>\n

Detection of Mycoplasma sp contamination in cell cultures using PCR technique<\/h4>\n

The DNA present in the culture supernatant samples is isolated by centrifugation. Aliquots of this extraction are used as template in a PCR reaction with a mixture of primers that allow the amplification of a conserved 16S rDNA sequence present in the vast majority of mycoplasma species listed so far in sequence databases. The result is revealed by agarose gel stained with DNA intercalant. As an internal control, another fragment is co-amplified to verify sensitivity and absence of Taq polymerase inhibitors. Samples are received during working days of the week until 17:00 hours. The results are delivered within 10 days. The report with the result is sent by e-mail or in printed version if necessary. <\/p>\n

 <\/p>\n

Cell-based assays: cytotoxicity, proliferation, transfection<\/h4>\n

We perform cell-based assays tailored to customer needs. These include cytotoxicity, proliferation, transfection, etc. assays. We have a wide collection of cell cultures from different origins to be used according to the model of interest. <\/p>\n

[\/dipi_advanced_tabs_item][dipi_advanced_tabs_item title=”Flow Cytometry” admin_label=”Citometr\u00eda de Flujo” img_container_max_width=”38%” content_image_align=”start” use_button=”on” at_button_text=”Contract” at_button_url=”mailto: vgorrostorrazo@pasteur.edu.uy, ubc@pasteur.edu.uy” at_button_url_new_window=”1″ tab_media=”none” font_icon=”\uf248||fa||400″ font_icon_active=”\uf248||fa||400″ _builder_version=”4.14.6″ _module_preset=”default” custom_button=”on” button_text_size=”14px” button_text_color=”#FFFFFF” button_bg_color=”#000000″ button_border_width=”0px” button_border_color=”#e39f15″ button_border_radius=”0px” button_font=”|600|||||||” button_use_icon=”on” button_icon=”||divi||400″ button_on_hover=”off” button_alignment=”left” button_custom_margin=”30px||||false|false” button_custom_padding=”10px|35px|10px|20px|false|false” hover_enabled=”0″ global_colors_info=”{}” button_bg_color__hover_enabled=”on|hover” button_border_color__hover_enabled=”on|desktop” button_border_color__hover=”#440099″ button_bg_color__hover=”rgba(68,0,153,0.8)” button_bg_enable_color__hover=”on” sticky_enabled=”0″]<\/p>\n

Access to conventional flow cytometers, continuous spectrum flow cytometers and cell sorters<\/h4>\n

<\/strong><\/p>\n

Assistance in experimental design, sample processing, data acquisition and analysis<\/h4>\n

<\/h4>\n

Isolation of different cell populations (up to 4 lanes) from a mixture by high-speed cell separation<\/h4>\n

<\/h4>\n

Cell cloning by deposition of a single cell per well using a cell sorter<\/h4>\n

<\/h4>\n

Quantification of cytokines in serum, plasma and cell culture supernatants, among others.<\/h4>\n

<\/h4>\n

Training and counseling for cytometry users<\/h4>\n

 <\/p>\n

Cell sorter:<\/strong> isolation of one or more cells (10,100,5,000,10,000, etc) in different devices: 6, 12, 48, 96 or 384-well plates, in 15 ml falcon or 5 ml tubes or directly to slides.<\/p>\n

Analytical cytometers:<\/strong> 2 with autosampler are available. They accept 48 and 96 well format (standard and deep well). Also 1.5, 2 and 5 mL tubes. <\/p>\n

[\/dipi_advanced_tabs_item][dipi_advanced_tabs_item title=”Hematological analysis of animal samples” admin_label=”An\u00e1lisis hematol\u00f3gico de muestras de origen animal” img_container_max_width=”38%” content_image_align=”start” use_button=”on” at_button_text=”Contract” at_button_url=”mailto: vgorrostorrazo@pasteur.edu.uy, ubc@pasteur.edu.uy” at_button_url_new_window=”1″ tab_media=”none” font_icon=”\uf248||fa||400″ font_icon_active=”\uf248||fa||400″ _builder_version=”4.14.6″ _module_preset=”default” custom_button=”on” button_text_size=”14px” button_text_color=”#FFFFFF” button_bg_color=”#000000″ button_border_width=”0px” button_border_color=”#e39f15″ button_border_radius=”0px” button_font=”|600|||||||” button_use_icon=”on” button_icon=”||divi||400″ button_on_hover=”off” button_alignment=”left” button_custom_margin=”30px||||false|false” button_custom_padding=”10px|35px|10px|20px|false|false” hover_enabled=”0″ global_colors_info=”{}” button_bg_color__hover_enabled=”on|hover” button_border_color__hover_enabled=”on|desktop” button_border_color__hover=”#440099″ button_bg_color__hover=”rgba(68,0,153,0.8)” button_bg_enable_color__hover=”on” sticky_enabled=”0″]<\/p>\n

Determination of hematological parameters of animal species with the Mindray BC5000Vet automatic equipment. 23 parameters are measured, including: white blood cells, lymphocytes, monocytes, neutrophils, basophils, eosinophils, red blood cells, Hb, hematocrit and platelets. <\/h4>\n

<\/h4>\n

This equipment allows the analysis of hematological parameters of 13 animal species: mouse, rat, dog, cat, monkey, horse, rabbit, pig, cow, sheep, llama, goat, ferret.<\/p>\n

Using flow cytometry and electrical impedance, it allows the measurement of up to 23 parameters: white blood cells, lymphocytes, monocytes, neutrophils, basophils, eosinophils, red blood cells, Hb, hematocrit, platelets among others (MCV, MCH, MCHC, RDW-CV, RDW-SD, MPV, PDW, PCT).<\/p>\n

The minimum sample volume is 15 \u00b5L of anticoagulated whole blood.
Anticoagulants accepted by the kit: EDTA-K3 or EDTA-K2.<\/p>\n

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Services of the Cell Biology Unit

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