ldecia@pasteur.edu.uy<\/a><\/p>\n[\/et_pb_team_member][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-investigacion” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
Research lines<\/h2>\n
[\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_padding=”27px|||||” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
Vector Suite and Artificial Binding Proteins (ABP)<\/strong>
Our laboratory has developed a set of vectors that allow the evaluation of different promoters and solubility-enhancing proteins through an efficient cloning strategy. This vector suite was extended in order to generate a set of vectors to include the evaluation of expression of recombinant proteins in different cellular compartments and cellular hosts. This helps to overcome the limitations encountered when working with a single subcellular location and a single type of host. In addition, these vectors also allow the evaluation of alternative purification strategies for the improvement of the yields of the target protein.<\/p>\n<\/p>\n
In the context of cancer therapeutic tools, our group recently focused on the generation of artificial binding proteins known as Affitins. This class of proteins presents a wide range of advantages compared to classical therapeutic antibodies that could be taken into account in the development of therapeutic approaches.
Compared to classical therapeutic antibodies, Aphitins are able to maintain high affinity constants even when their molecular weight remains small. This could be very useful in lymphoid neoplasms, in order to gain access to solid tissues as secondary lymphoid organs, where leukemic cells receive survival signals that acquire favourable conditions of proliferation. In this line, a new generation of combinatorial protein engineering technologies has recently been created in our laboratory. The results have made it possible to propose the use of these artificial binding proteins as versatile selective glycosidase inhibitors and, potentially, as enzymatic inhibitors in general, which could be foreseen for future tumour therapy strategies (Correa et al., PlosOne, 2014).<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-equipos” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
Main equipment<\/h2>\n
[\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_padding=”27px|||||” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
\n- \u00c4KTAxpress \/ \u00c4KTA Pure and \u00c4KTA Purifier<\/span>:<\/li>\n<\/ul>\n
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![\"\"](\"https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/02\/AKTAxpress_Portada-167x300.jpg\")
![\"\"](\"https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/02\/AKTAPurifier_Portada-300x201.jpg\")
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\n- Benchtop Bioreactor BIOSTAT\u00ae B plus (Prokaryotic culture)<\/span>:<\/li>\n<\/ul>\n
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![\"\"](\"https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/02\/Benchtop_Portada-300x201.jpg\")
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[\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_margin=”70px||||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
\n- CelliGen 310 Bioreactor (Eukaryotic culture)<\/span>:<\/li>\n<\/ul>\n
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![\"\"](\"https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/02\/CelliGen_Portada-300x201.jpg\")
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\n- BelloCell 3000 Bioreactor (Eukaryotic culture)<\/span><\/li>\n
- EmulsiFlex-C5 Homogenizer<\/span><\/li>\n
- Multitron 2 Incubated Shaker<\/span>:<\/li>\n<\/ul>\n
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![\"\"](\"https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/02\/Multitron_Portada-300x201.jpg\")
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[\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
Services<\/h2>\n
[\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_padding=”27px|||||” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
\n- Expression of recombinant proteins (RP) in prokaryotic and eukaryotic systems:
– Expression of RP in E. coli
– Expression of RP in mammalian cells<\/li>\n<\/ul>\n<\/p>\n
\n- Optimization of the expression of soluble recombinant proteins<\/li>\n
- Refolding and soluble production of bacterial inclusion bodies<\/li>\n
- Maintenance of collections of expression vectors and bacterial strains<\/li>\n<\/ul>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-cursos” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
Courses<\/h2>\n
[\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_padding=”27px|||||” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
\n- \u201cIntegrating IP Montevideo technologies\u201d. Course for researchers and technicians of IP Montevideo, 2017.<\/li>\n
- Postgraduate course: \u201cIntroduction to the structural and functional analysis of proteins\u201d, Institut Pasteur de Montevideo. September-November 2014.<\/li>\n
- Postgraduate course: \u201cExpression, Purification and Crystallization of Recombinant Proteins by High-throughput Methodologies\u201d. February 2013.<\/li>\n
- Postgraduate course: \u201cExpression of Recombinant Proteins\u201d. PEDECIBA-Biology- MSc in Biotechnology, Faculty of Science \u2013 Institut Pasteur de Montevideo. Nov5\u2013Dec10, 2008.<\/li>\n<\/ul>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-proyectos” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
Projects<\/h2>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-proyectos” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” custom_margin=”0px||0px||false|false” custom_padding=”0px||0px||false|false” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_margin=”||0px||false|false” custom_padding=”0px||0px||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
FCE_3_2016_1_125765<\/strong> Design and development of artificial binding proteins with potential use in biomedicine. Scientific responsible: Agust\u00edn Correa.<\/p>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-publicaciones” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||26px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n
Main publications<\/h2>\n
[\/et_pb_text][et_pb_accordion icon_color=”#005293″ _builder_version=”4.16″ _module_preset=”default” toggle_text_color=”#005293″ toggle_font=”Titillium Web|600|||||||” toggle_font_size=”17px” custom_margin=”||||false|false” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_accordion_item title=”vacio” open=”on” _builder_version=”4.16″ _module_preset=”default” custom_css_main_element=”display:none” global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_accordion_item][et_pb_accordion_item title=”2022″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content” open=”off”]<\/p>\n