{"id":4860,"date":"2022-03-25T13:32:11","date_gmt":"2022-03-25T13:32:11","guid":{"rendered":"https:\/\/pasteur.uy\/uncategorized\/analytical-biochemistry-and-proteomics\/"},"modified":"2026-04-10T13:44:27","modified_gmt":"2026-04-10T16:44:27","slug":"analytical-biochemistry-and-proteomics","status":"publish","type":"post","link":"https:\/\/pasteur.uy\/en\/units\/analytical-biochemistry-and-proteomics\/","title":{"rendered":"Analytical Biochemistry and Proteomics"},"content":{"rendered":"

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Analytical Biochemistry and Proteomics<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ _module_preset=”default” max_width=”80%” custom_padding=”||49px|||” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

The Analytical Biochemistry and Proteomics Unit (Ubypa) \u2014an IP Montevideo and Instituto de Investigaciones Biol\u00f3gicas Clemente Estable (IIBCE) Mixed Unit\u2014 has the objective of carrying out and supporting biomedical research projects based on mass spectrometry (MS) and proteomics. It also offers training, scientific assistance and access to EM-based proteomic technologies to the local scientific community; and contributes to local and regional education programs in this area.<\/p>\n

<\/p>\n

During the last years, the Unit incorporated mass spectrometers and developed the know-how to expand the quality and type of analytical procedures available.<\/p>\n

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Currently, our analytical portfolio includes \u201cshotgun\u201d proteomic strategies, as well as gel-based strategies; in vivo and in vitro interactome studies; and analysis of post-translational protein modifications.<\/p>\n

<\/p>\n

The research projects of our group focus on the study of signaling mechanisms in mycobacteria using proteomic approaches, with emphasis on the analysis of protein phosphorylation. In particular, we are interested in understanding some key processes for pathogenic mycobacteria, such as Mycobacterium tuberculosis, which are related to their ability to survive inside the host.<\/p>\n

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theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Members<\/h2>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_4,1_4,1_4,1_4″ use_custom_gutter=”on” gutter_width=”2″ module_class=”dfc-integrantes” _builder_version=”4.16″ _module_preset=”default” width_tablet=”” width_phone=”auto” width_last_edited=”on|phone” max_width=”80%” custom_padding=”||6px|||” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”1_4″ module_class=”dfc-humano” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Rosario Dur\u00e1n, PhD” position=”Head ” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/01\/Duran-Rosario.jpg” _builder_version=”4.16″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” link_option_url=”https:\/\/pasteur.uy\/en\/heads-of-labs-and-units\/rosario-duran\/” link_option_url_new_window=”on” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

\n duran@pasteur.edu.uy<\/a>\n <\/p>\n

[\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Magdalena Portela, BSc” position=”Senior associate technician” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/02\/Portela-Magdalena.jpg” _builder_version=”4.16″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

\n madelon@pasteur.edu.uy<\/a>\n <\/p>\n

[\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ module_class=”dfc-humano” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Anal\u00eda Lima, PhD” position=”Senior associate researcher” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/02\/Lima-Analia.jpg” _builder_version=”4.16″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

\n alima@pasteur.edu.uy<\/a>\n <\/p>\n

[\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Alejandro Leyva, BSc” position=”Senior Associate Technician” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2024\/11\/Alejandro-Leyva-2.jpg” _builder_version=”4.27.4″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” custom_css_member_description=”letter-spacing: -0.5px !important;” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

PhD student
alejandrolp@pasteur.edu.uy<\/a><\/p>\n

[\/et_pb_team_member][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_4,1_4,1_4,1_4″ use_custom_gutter=”on” gutter_width=”2″ module_class=”dfc-integrantes” _builder_version=”4.16″ _module_preset=”default” width_tablet=”” width_phone=”auto” width_last_edited=”on|phone” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”1_4″ module_class=”dfc-humano” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Flavio Pazos Obreg\u00f3n, PhD” position=”Investigador adjunto senior” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2024\/08\/Flavio-Pazos-.jpg” _builder_version=”4.27.4″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

\n flavio.pazos@gmail.com<\/a>\n <\/p>\n

[\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Marlon D. M. Santos, PhD” position=”Investigador adjunto senior” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2023\/06\/Santos-Marlon.jpg” _builder_version=”4.27.4″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

ICC\/Fiocruz
marlondms@pasteur.edu.uy<\/a><\/p>\n

[\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ module_class=”dfc-humano” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Paulo Carvalho, PhD” position=”Associate researcher – Fiocruz Paran\u00e1” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/09\/Carvalho-Paulo.jpg” _builder_version=”4.16″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” custom_css_member_description=”letter-spacing: -0.5px !important;” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”][\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Azalia Rodr\u00edguez, MSc” position=”Deputy technitian” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2022\/02\/Rodriguez-Azalia.jpg” _builder_version=”4.16″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” custom_css_member_description=”letter-spacing: -0.5px !important;” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

\n <\/a>
\n arodriguez@pasteur.edu.uy<\/a>
\n <\/a>\n <\/p>\n

[\/et_pb_team_member][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_4,1_4,1_4,1_4″ use_custom_gutter=”on” gutter_width=”2″ module_class=”dfc-integrantes” _builder_version=”4.27.0″ _module_preset=”default” width_tablet=”” width_phone=”auto” width_last_edited=”on|phone” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”1_4″ module_class=”dfc-humano” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Emilia Torriglia” position=”Intern” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2025\/05\/Emilia-Torriglia-.jpg” _builder_version=”4.27.4″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

etorriglia@pasteur.edu.uy<\/a><\/p>\n

[\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Nazareno Cabrera” position=”Intern” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2025\/10\/Nazareno-Cabrera.png” _builder_version=”4.27.4″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

ncabrera@pasteur.edu.uy<\/a><\/p>\n

[\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_team_member name=”Alfonso Taboada” position=”Research Assistant” image_url=”https:\/\/pasteur.uy\/wp-content\/uploads\/2025\/04\/Alfonso-Taboada.jpg” _builder_version=”4.27.4″ _module_preset=”default” header_font=”|700|||||||” body_font=”|300|||||||” position_font=”|600|||||||” position_text_color=”#005293″ position_line_height=”25px” animation_style=”fade” global_colors_info=”{}” position__hover_enabled=”off|desktop” theme_builder_area=”post_content”]<\/p>\n

ataboada@pasteur.edu.uy<\/a><\/p>\n

[\/et_pb_team_member][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-investigacion” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Research lines<\/h2>\n

[\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_padding=”27px|||||” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Characterization of processes regulated by protein phosphorylation in Mycobacterium tuberculosis<\/strong>
Mycobacterium tuberculosis uses protein phosphorylation at hydroxylated residues to regulate vital processes. In the past, we described the regulatory mechanisms of Ser\/Thr kinases and identified some of their substrates and targets \u201cdownstream\u201d in the signaling pathways. At present, we are using in vivo cross-linking in combination with mass spectrometry to obtain a \u201csnapshot\u201d of the protein-protein interactions in the living bacteria, with emphasis in the study of the interactome of the previously identified kinase-substrates. This has allowed us to begin to elucidate phosphorylation-dependent interactions that participate in the regulation of nitrogen uptake and cell division.<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-equipos” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Main equipment<\/h2>\n

[\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_padding=”27px|||||” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

    \n
  • HPLC, Agilent 1200<\/li>\n
  • Capilar HPLC, Agilent 1200<\/li>\n
  • Nano HPLC, Easy-nLC 1000, Thermo<\/li>\n
  • Nano HPLC Ultimate 300, Thermo<\/li>\n
  • 2D Electrophoresis, EttanIPGphor + EttanDaltSix<\/li>\n
  • Typhoon FLA 9500, GE Healthcare<\/li>\n
  • 4800 MALDI TOF\/TOF Mass Spectrometer, Abi Sciex<\/li>\n
  • LTQ Velos + ETD Mass Spectrometer, Thermo<\/li>\n
  • Q-exactive (Q-Orbitrap), Thermo<\/li>\n
  • Microfraction collector for MALDI plating, Probot LC-Packing<\/li>\n<\/ul>\n

    [\/et_pb_text][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

    Services<\/h2>\n

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    IDENTIFICATION OF BACTERIA<\/span><\/h3>\n

    <\/p>\n

    By mass spectrometry:shotgun proteomic methodology for the identification of bacteria in clinical isolates, especially in those cases in which identification by routine methods could not be achieved.<\/span><\/p>\n

    <\/p>\n

    <\/span><\/p>\n

    <\/p>\n

    CONFIRMATION OF RECOMBINANT PROTEIN SEQUENCES <\/span><\/h3>\n

    <\/p>\n

    By spectrometry:<\/span> LC-MS\/MS mass spectrometry<\/span> from samples from SDS-PAGE or in solution and database search.<\/p>\n

    <\/p>\n

    <\/p>\n

    PROTEOMIC PROFILING ANALYSIS<\/span><\/h3>\n

    <\/p>\n

    By NanoLC-MS\/MS: Shotgun proteomics methodology for the identification of proteomic profiles of organisms with sequenced genomes.<\/span><\/p>\n

    <\/p>\n

    <\/span><\/p>\n

    <\/p>\n

    COMPARATIVE QUANTITATIVE PROTEOMICS<\/span><\/h3>\n

    <\/p>\n

    By NanoLC-MS\/MS with or without labeling:<\/span>shotgun proteomics methodology<\/span>for the comparison of proteomic profiles obtained from two or more biological conditions.<\/p>\n

    <\/p>\n

    <\/p>\n

    INTERACTOME ANALYSIS <\/span><\/h3>\n

    <\/p>\n

    By chemical crosslinking and proximity marking<\/p>\n

    <\/p>\n

    <\/p>\n

    ANALYSIS OF POST-TRANSLATIONAL MODIFICATIONS<\/span><\/h3>\n

    <\/p>\n

    By NanoLC-MS\/MS: identification of post-translational modifications by shotgun proteomics and database search.<\/span><\/p>\n

    [\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-servicios” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

    Courses<\/h2>\n

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      \n
    • ICGEB & UNU-BIOLAC \u201cProteome Analysis by Mass Spectrometry\u201d; October 15-23, 2018. Organizers: Rosario Dur\u00e1n, Paulo Carvalho & Carlos Batthy\u00e1ny.<\/li>\n
    • UNU-BIOLAC & PEDECIBA \u201cProteome Analysis by Mass Spectrometry\u201d. November 28 – December 2, 2016. Organizers: Rosario Dur\u00e1n, Paulo Carvalho & Carlos Batthy\u00e1ny.<\/li>\n
    • UNU-BIOLAC & RIIP (Institut Pasteur International Network) \u201cProteome Analysis by Mass Spectrometry\u201d. September 1-12, 2014. Organizers: Rosario Dur\u00e1n & Carlos Batthy\u00e1ny.<\/li>\n
    • UNU-Biolac- \u201cMass Spectrometry (MS) in Proteomics\u201d Institut Pasteur de Montevideo. November 26 – December 8, 2012. Organizers: C. Batthyany, R. Dur\u00e1n<\/li>\n
    • EMBO World Practical Course. \u201cMass Spectrometry in Protein Analysis and Characterization\u201d, EMBO, IPMONT, ANII; Montevideo, Uruguay. March 16-26, 2010. Organizers: C. Cerve\u00f1ansky, R. Dur\u00e1n & C. Batthyany<\/li>\n
    • \u201cWorkshop on mass spectrometry and it\u2019s application on protein analysis\u201d, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica Faculdade de Ci\u00eanciasFarmac\u00eauticas de Ribeir\u00e3o Preto Universidade de, S\u00e3o Paulo, Brasil. October 19-24, 2009. Organizers: R. Dur\u00e1n & C. Batthyany.<\/li>\n
    • Course \u201cIntegrating IP Montevideo technologies\u201d. Module: \u201cProtein Characterization using Mass Spectrometry\u201d. October 6, 2017. Organizers: A. Lima and M. Portela.<\/li>\n<\/ul>\n

      [\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-proyectos” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

      Projects<\/h2>\n

      [\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ module_class=”dfc-proyectos” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” custom_margin=”0px||0px||false|false” custom_padding=”0px||0px||false|false” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_margin=”||0px||false|false” custom_padding=”0px||0px||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

      2020-2023<\/strong> \u2013 <\/strong>Descifrando la arquitectura molecular del elongasoma y divisoma de Corynebacterineae mediante marcado por proximidad en la c\u00e9lula viva. Fondo Clemente Estable FCE_1_2019_1_155569. ANII. Responsible: Rosario Dur\u00e1n.<\/p>\n

      <\/p>\n

      2020-2022<\/strong> \u2013 <\/strong>Adaptation of pathogenic Leptospira to oxidative stress: its contribution to virulence and host colonization Programmes Transversaux de Recherche \u2013 PTR. Coordinator: Dr. Benaroudj Nadia. Local coordinator: Rosario Dur\u00e1n.<\/p>\n

      <\/p>\n

      2018-2023 \u2013 <\/strong>Molecular mechanisms of phospho-dependent regulation and assembly of the bacterial divisome. ANR-Agence Nationale de la Recherche, Francia. 2018-2023. Coordinador del consorcio: Dr. PM Alzari. Local coordinator: Rosario Dur\u00e1n.<\/p>\n

      <\/p>\n

      2015-2018<\/strong> \u2013 FHA domain mediated signaling cascades in mycobacteria and its role in the adaptation to the host environment. Responsible:Rosario Dur\u00e1n.Fondo Clemente Estable, Modalidad I. FCE_1_2014_1_104045.<\/p>\n

      [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” custom_margin=”3px|||||” custom_padding=”0px||||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

      2014-2016<\/strong> \u2013 Comparative proteomic analysis of two Pseudomonas aeruginosa strains showing differential adhesivity to epithelial cells. Responsible: J. Rossello Fondo Clemente Estable, Modalidad II. FCE_3_2013_1_100344.<\/p>\n

      <\/p>\n

      2014-2016<\/strong> \u2013 Towards the elucidation of the molecular mechanism used by PknG to exert its role as virulence factor. Responsible: M. Gil. Fondo Clemente Estable, Modalidad II. FCE_3_2013_1_100358.<\/p>\n

      <\/p>\n

      2012-2014<\/strong> \u2013 Exploring the role of mosquito\u2019s saliva in the transmission of Rift Valley fever; Actions Concert\u00e9es Interpasteuriennes (ACIP). Coordinator: V. CHOUMET (Paris). Responsibles in Uruguay: C. Batthy\u00e1ny & R. Dur\u00e1n.<\/p>\n

      [\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=”dfc-publicaciones” _builder_version=”4.16″ _module_preset=”default” max_width=”80%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.16″ _module_preset=”default” header_2_font=”|700|||||||” header_2_text_color=”#005293″ header_2_font_size=”35px” custom_margin=”||0px||false|false” custom_padding=”||26px||false|false” header_2_font_size_tablet=”35px” header_2_font_size_phone=”29px” header_2_font_size_last_edited=”on|phone” saved_tabs=”all” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

      Main publications<\/h2>\n

      [\/et_pb_text][et_pb_accordion icon_color=”#005293″ _builder_version=”4.16″ _module_preset=”default” toggle_text_color=”#005293″ toggle_font=”Titillium Web|600|||||||” toggle_font_size=”17px” custom_margin=”||||false|false” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_accordion_item title=”vacio” open=”on” _builder_version=”4.16″ _module_preset=”default” custom_css_main_element=”display:none” global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_accordion_item][et_pb_accordion_item title=”2021″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content” open=”off”]<\/p>\n

        \n
      • Lima A, Leyva A, Rivera B, Portela MM, Gil M, Cascioferro A, Lisa MN, Wehenkel A, Bellinzoni M, Carvalho PC, Batthy\u00e1ny C, Alvarez MN, Brosch R, Alzari PM, Dur\u00e1n R. (2021) Proteome remodeling in the Mycobacterium tuberculosis PknG knockout: Molecular evidence for the role of this kinase in cell envelope biogenesis and hypoxia response. Journal of Proteomics. 244, 104276 DOI: 10.1016\/j.jprot.2021.104276<\/li>\n
      • Lisa MN, Sogues A, Barilone N, BaumgartM3, Gil M, Gra\u00f1a M, Dur\u00e1n R, Biondi RM, Bellinzoni M, Bott M, Alzari PM (2021) A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG. mBio e0171721. doi: 10.1128\/mBio.01717-21.<\/li>\n
      • Martinez-Sanguin\u00e9 AY, D\u2019Alessandro B, Langleib M, Traglia GM, M\u00f3naco A, Dur\u00e1n R, Chabalgoity JA, Betancor L, Yim L. (2021) Salmonella enteric Serovars Dublin and Enteritidis Comparative Proteomics Reveals Differential Expression of Proteins Involved in Stress Resistance, Virulence, and Anaerobic Metabolism. Infect Immun. 16, 89(3). doi: 10.1128\/IAI.00606-20.<\/li>\n
      • Ott C, Tomasina F, Campolo N, Bartesaghi S, Mastrogiovanni M, Leyva A, Batthyany C, Meinl W, Grune T, Radi R. (2021) Decreased proteasomal cleavage at nitrotyrosine sites in proteins and peptides. Redox Biology. 46, 102106 DOI: 10.1016\/j.redox.2021.102106<\/li>\n
      • Gonz\u00e1lez LJ, Encinosa Guzm\u00e1n PE, Machado W, Pousa S, Leyva A, Cano Arguelles AL, Cabrera G, Espinosa LA, Parra R, Hern\u00e1ndez R, Bello Soto Y, Ledesma FL, Joglar M, Guirola O, Ulrich Kurt L, Carvalho PC, Cabrales A, Garay H, Besada V, Dur\u00e1n R, Takao T, Estrada MP, Rodr\u00edguez-Mallon A. (2021). Synthesis, LC-MS\/MS analysis, and biological evaluation of two vaccine candidates against ticks based on the antigenic P0 peptide from R. sanguineus linked to the p64K carrier protein from Neisseria meningitidis. Anal Bioanal Chem. 413, 5885\u20135900 DOI: 10.1007\/s00216-021-03569-0<\/li>\n
      • Cardozo V, Vaamonde L, Parodi-Talice A, Zuluaga MJ, Agrati D, Portela M, Lima A, Blasina F, Dajas F, Bed\u00f3 G. (2021) Multitarget neuroprotection by quercetin: Changes in gene expression in two perinatal asphyxia models. Neurochem Int. 147:105064. doi: 10.1016\/j.neuint.2021.105064.<\/li>\n
      • Chalar C, Clivio G, Montagne J, Cost\u00e1bile A, Lima A, Papa NG, Berois N, Arezo MJ. (2021) Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III..PLoS One. 16(6):e0251820. doi: 10.1371\/journal.pone.0251820.<\/li>\n
      • Demicheli V, Tomasina F, Sastre S, Zeida A, T\u00f3rtora V, Lima A, Batthy\u00e1ny C, Radi R. (2021) Cardiolipin interactions with cytochrome c increase tyrosine nitration yields and site-specificity. Arch Biochem Biophys. 703:108824. doi: 10.1016\/j.abb.2021.108824.<\/li>\n<\/ul>\n

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          \n
        • Rivera B, Leyva A, Portela MM, Moratorio G, Moreno P, Dur\u00e1n R, Lima A. (2020) Quantitative proteomic dataset from oro- and naso-pharyngeal swabs used for COVID-19 diagnosis: Detection of viral proteins and host\u2019s biological processes altered by the infection. Data in Brief. 32, 106121 DOI: 10.1016\/j.dib.2020.106121<\/li>\n
        • Sogues A, Martinez M, Gaday Q, Ben Assaya M, Gra\u00f1a M, Voegele A, VanNieuwenhze M, England P, Haouz A, Chenal A, Tr\u00e9pout S, Duran R, Wehenkel AM, Alzari PM. (2020) Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum Nature Communications 11, 1641 doi:10.1038\/s41467-020-15490-8.<\/li>\n
        • Rodr\u00edguez Mall\u00f3n A, Javier Gonz\u00e1lez L, Encinosa Guzm\u00e1n PE, Bechara GH, Sanches GS, Pousa S, Cabrera G, Cabrales A, Garay H, Mej\u00edas R, L\u00f3pez \u00c1lvarez JR, Bello Soto Y, Almeida F, Guirola O, Rodr\u00edguez Fern\u00e1ndez R, Fuentes Castillo A, M\u00e9ndez L, Jim\u00e9nez S, Licea-Navarro A, Portela M, Dur\u00e1n R, Estrada MP (2020) Functional and Mass Spectrometric Evaluation of an Anti-Tick Antigen Based on the P0 Peptide Conjugated to Bm86 Protein. Pathogens 25;9(6):513. doi:10.3390\/pathogens9060513.<\/li>\n
        • Bei J, Bigi MM, Lima A, Zhang Q, Blanco FC, Lopez B, Yu T, Wang Z, Dai Z,Chen Z, Cataldi AA, Sasiani MC, Ritacco V, de la Barrera S, Soria MA, Dur\u00e1n R, Bigi F. (2020) A Phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of Mycobacterium tuberculosis with opposite epidemiological fitness. Biomed Res Int. 8; 2020:4741237. 6doi: 10.1155\/2020\/4741237.<\/li>\n
        • Forrellad MA, Blanco FC, Marrero Diaz De Villegas R, V\u00e1zquez CL, Yaneff A, Garc\u00eda EA, Gutierrez M, Dur\u00e1n R, Villarino A, Bigi F. (2020). Rv2577 of Mycobacterium tuberculosis is a virulence factor with dual phosphatase and phosphodiesterase functions. Front. Microbiol. 11:570794. doi: 10.3389\/fmicb.2020.570794.<\/li>\n
        • Carre\u00f1o M, Bresque M, Machado M, Santos L, Dur\u00e1n R, Vitturi DA Escande C, Denicola A (2020). Nitro-fatty acids as activators of hSIRT6 deacetylase activity. Journal of Biological Chemistry 295, 18355 doi:10.1074\/jbc.RA120.014883.<\/li>\n
        • Colman L, Caggiani M, Leyva A, Bresque M, Liechocki S, Maya-Monteiro CM, Mazal D, Batthyany C, Calliari A, Contreras P, Escande C. (2020) The protein Deleted in Breast Cancer-1 (DBC1) regulates vascular response and formation of aortic dissection during Angiotensin II infusion. Sci Rep. 10, 6772 DOI: 10.1038\/s41598-020-63841-8.<\/li>\n
        • Mosquillo MF, Smircich P, Lima A, Gehrke SA, Scalese G, Machado I, Gambino D, Garat B, P\u00e9rez-D\u00edaz L. (2020) High Throughput Approaches to Unravel the Mechanism of Action of a New Vanadium-Based Compound against Trypanosoma cruzi. Bioinorg Chem Appl. 11:1634270. doi: 10.1155\/2020\/1634270.<\/li>\n
        • Mosquillo MF, Smircich P, Ciganda M, Lima A, Gambino D, Garat B, P\u00e9rez-D\u00edaz L. (2020) Comparative high-throughput analysis of the Trypanosoma cruzi response to organometallic compounds. Metallomics. 27;12(5):813-828. doi: 10.1039\/d0mt00030b.<\/li>\n
        • Tucci P, Portela M, Rivas Chetto C, Gonz\u00e1lez-Sapienza G, Mar\u00edn M.(2020). Integrative proteomic and glycoproteomic profiling of Mycobacterium tuberculosis culture filtrate. Plos One 15(3):e0221837. doi: 10.1371\/journal.pone.0221837. eCollection 2020<\/li>\n<\/ul>\n

          [\/et_pb_accordion_item][et_pb_accordion_item title=”2019″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content” open=”off”]<\/p>\n

            \n
          • Gil M, Lima A, Rivera B, Rossello J, Urd\u00e1niz E, Cascioferro A, Carri\u00f3n F, Wehenkel A, Bellinzoni M, Batthy\u00e1ny C, Pritsch O, Denicola A, Alvarez MN, Carvalho PC, Lisa MN, Brosch R, Piuri M, Alzari PM, Dur\u00e1n R. (2019) New substrates and interactors of the mycobacterial Serine\/Threonine protein kinase PknG identified by a tailored interactomic approach. (2019) J Proteomics. 192, 321-333. doi: 10.1016\/j.jprot.2018.09.013.<\/li>\n
          • Bellinzoni M, Wehenkel AM, Dur\u00e1n R, Alzari PM. (2019) Novel mechanistic insights into physiological signaling pathways mediated by mycobacterial Ser\/Thr protein kinases. Microbes Infect. 21(5-6):222-229. doi: 10.1016\/j.micinf.2019.06.015. Review.<\/li>\n
          • Santos L, Colman L, Contreras P, Chini CC, Carlomagno A, Leyva A, Bresque M, Marmisolle I, Quijano C, Dur\u00e1n R, Irigo\u00edn F, Prieto-Echag\u00fce V, Vendelbo MH, Sotelo-Silveira JR, Chini EN, Badano JL, Calliari AJ, Escande C. (2019) A novel form of Deleted in breast cancer 1 (DBC1) lacking the N-terminal domain does not bind SIRT1 and is dynamically regulated in vivo. Sci Rep. 9, 14381 DOI: 10.1038\/s41598-019-50789-7.<\/li>\n
          • Miles S, Portela M, Cyrklaff M, Ancarola ME, Frischknecht F, Dur\u00e1n R, Dematteis S, Mourglia\u2010Ettlin G (2019) Combining proteomics and bioinformatics to explore novel tegumental antigens as vaccine candidates against Echinococcus granulosus infection. J Cell Biochem. 120(9),15320-15336. doi: 10.1002\/jcb.28799.<\/li>\n
          • Spinelli R, Sanchis I, Aimaretti FM, Attademo AM, Portela M, Humpola MV, Tonarelli GG, Siano AS. (2019) Natural Multi-Target Inhibitors of Cholinesterases and Monoamine Oxidase Enzymes with Antioxidant Potential from Skin Extracts of Hypsiboas cordobae and Pseudis minuta (Anura: Hylidae). ChemBiodivers 16(1):e1800472. doi:10.1002\/cbdv.201800472.<\/li>\n
          • Chavarr\u00eda C, Trostchansky A, Dur\u00e1n R, Rubbo H, Souza JM. (2019) Nitroalkylation of \u03b1-Synuclein by Nitro-Oleic Acid: Implications for Parkinson\u2019s Disease. AdvExp Med Biol. 2019; 1127:169-179. doi: 10.1007\/978-3-030-11488-6_11. Review.<\/li>\n
          • Ramos-Artuso F, Galatro A, Lima A, Batthy\u00e1ny C, Simontacchi M. (2019) Early events following phosphorus restriction involve changes in proteome and affects nitric oxide metabolism in soybean leaves. Environ Exp Bot. 161:203-2017. https:\/\/doi.org\/10.1016\/j.envexpbot.2019.01.002. <\/li>\n<\/ul>\n

            [\/et_pb_accordion_item][et_pb_accordion_item title=”2018″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content” open=”off”]<\/p>\n

              \n
            • Pi\u00f1as GE, Reinoso-Vizcaino NM, Yandar Barahona NY, Cortes PR, Duran R, Badapanda C, Rathore A, Bichara DR, Cian MB, Olivero NB, Perez DR, Echenique J. 2018. Crosstalk between the serine\/threonine kinase StkP and the response regulator ComE controls the stress response and intracellular survival of Streptococcus pneumoniae. PLoS Pathog. 14(6):e1007118<\/li>\n<\/ul>\n

              [\/et_pb_accordion_item][et_pb_accordion_item title=”2017″ _builder_version=”4.16″ _module_preset=”default” link_option_url_new_window=”on” global_colors_info=”{}” theme_builder_area=”post_content” open=”off”]<\/p>\n

                \n
              • Fl\u00f3 M., Margenat M., Pellizza L., Gra\u00f1a M., Dur\u00e1n R., B\u00e1ez A., Salceda E., Soto E., Alvarez B., Fern\u00e1ndez C. 2017. Functional diversity of secreted cestodeKunitz proteins: Inhibition of serine peptidases and blockade of cation channels. PLoSPathogens. 13(2):e1006169<\/li>\n
              • Prieto D., Sotelo N., Seija N., Sernbo S., Abreu C., Dur\u00e1n R., Gil M., Sicco E., Irigoin V., Oliver C., Landoni A.I., Gabus R., Dighiero G., Oppezzo P. 2017. S100-A9 protein in exosomes from chronic lymphocytic leukemia cells promotes NF-\u03baB activity during disease progression. Blood. 130(6):777-788<\/li>\n
              • Silva A.R.F., Lima D.B., Leyva A., Duran R., Batthyany C., Aquino P.F., Leal J.C., Rodriguez J.E., Domont G.B., Santos M.D.M., Chamot-Rooke J., Barbosa V.C., Carvalho P.C. 2017 DiagnoProt: a tool for discovery of new molecules by mass spectrometry. Bioinformatics. 33(12):1883-1885. doi: 10.1093\/bioinformatics\/btx093<\/li>\n
              • Silva A.R.F., Lima D.B., Leyva A., Duran R., Batthyany C., Aquino P.F., Leal J.C., Rodriguez J.E., Domont G.B., Santos M.D.M., Chamot-Rooke J., Barbosa V.C., Carvalho P.C. 2017 DiagnoProt: a tool for discovery of new molecules by mass spectrometry. Bioinformatics. 33(12):1883-1885. doi: 10.1093\/bioinformatics\/btx093<\/li>\n
              • Cabrera G., Lundberg U., Rodr\u00edguez-Ulloa A., Herrera M., Machado W., Portela M., Palomares S., Espinosa L.A., Ramos Y., Dur\u00e1n R., Besada V., Vonasek E., Gonz\u00e1lez L.J. 2017 Protein content of the Hylesiametabus egg nest setae (Cramer [1775]) (Lepidoptera: Saturniidae) and its association with the parental investment for the reproductive success and lepidopterism. J Proteomics. 150:183-200<\/li>\n
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                      [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

                      Analytical Biochemistry and ProteomicsThe Analytical Biochemistry and Proteomics Unit (Ubypa) \u2014an IP Montevideo and Instituto de Investigaciones Biol\u00f3gicas Clemente Estable (IIBCE) Mixed Unit\u2014 has the objective of carrying out and supporting biomedical research projects based on mass spectrometry (MS) and proteomics. It also offers training, scientific assistance and access to EM-based proteomic technologies to the […]<\/p>\n","protected":false},"author":8,"featured_media":3064,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[59],"tags":[],"dipi_cpt_category":[],"class_list":["post-4860","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-units"],"acf":[],"_links":{"self":[{"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/posts\/4860","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/comments?post=4860"}],"version-history":[{"count":5,"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/posts\/4860\/revisions"}],"predecessor-version":[{"id":24854697,"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/posts\/4860\/revisions\/24854697"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/media\/3064"}],"wp:attachment":[{"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/media?parent=4860"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/categories?post=4860"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/tags?post=4860"},{"taxonomy":"dipi_cpt_category","embeddable":true,"href":"https:\/\/pasteur.uy\/en\/wp-json\/wp\/v2\/dipi_cpt_category?post=4860"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}