- PhD in Biological Sciences. PEDECIBA (UdelaR – MEC) (2012 – 2016): “Small regulatory RNAs in the extracellular milieu: secretion, stability, transport and potential in intercellular communication”.
- Master in Biological Sciences, UdelaR (2009 – 2011).
- Degree in Biochemistry, Facultad de Ciencias, UdelaR (2004 – 2008).
-
Non-coding RNAs
-
Extracellular RNA
-
RNA biochemistry
-
Analytical Biochemistry
- Castellano M, Blanco V, Li Calzi M, Costa B, Witwer K, Hill M, Cayota A, Segovia M, Tosar JP (2024). Ribonuclease activity undermines immune sensing of naked extracellular RNA. BioRxiv pre-print. DOI: 10.1101/2024.04.23.590771.
- Tosar JP, Castellano M, Costa B, Cayota A (2024). Small RNA structural biochemistry in a post-sequencing era. Nature Protocols 19, 595-602.
- Costa B, Li Calzi M, Castellano M, Blanco V, Cuevasanta E, Litvan I, Ivanov P, Witwer K, Cayota A, Tosar J.P. (2023). Nicked tRNAs are stable reservoirs of tRNA halves in cells and biofluids. Proceedings of the National Academy of Sciences USA (PNAS) 120 (4), e2216330120.
- Tosar, J.P.; Witwer, K.; Cayota, A. (2021) Revisiting extracellular RNA release, processing and function. Trends in Biochemical Sciences 46(6): 438-445.
- Tosar, J.P.; Segovia, M.; Gámbaro, F.; Akiyama, Y.; Fagúndez, P.; Costa, B.; Possi, T.; Hill, M.; Ivanov, P.; Cayota, A. (2020) Fragmentation of extracellular ribosomes and tRNAs shapes the extracellular RNAome. Nucleic Acids Research 48(22): 12874-12888.
- Tosar, J.P.; Gámbaro, F.; Darré, L.; Pantano, S.; Westhof, E.; Cayota, A. (2018) Dimerization confers increased stability to nucleases in extracellular 5′ halves from glycine and glutamic acid tRNAs. Nucleic Acids Research 46 (17): 9081-9093.
- Tosar J.P.; Rovira, C.; Cayota, A. (2018) Non-coding RNA fragments account for the majority of annotated piRNAs expressed in somatic non-gonadal tissues. Communications Biology 1, e2
- Doldán, X; Fagúndez, P.; Cayota, A.; Laíz, J.; Tosar, J.P. (2016) Electrochemical sandwich immunosensor for determination of exosomes based on surface marker-mediated signal amplification. Analytical Chemistry 88 (21): 10466-10473.