Alejandro Buschiazzo

Alejandro Buschiazzo, PhD

Molecular and Structural Microbiology Laboratory

Protein Crystallography Unit

  • BSC in Biological Science, Faculty of Natural Science, Universidad Nacional de La Plata, Argentina (1991).
  • PhD in Chemical Science, Leloir Institute, Universidad de Buenos Aires, Argentina (1999).
  • Posdoc in Structural Biology of Trypanosomatid Sialidases, Institut Pasteur, France (1999-2003).
  • Protein biochemistry
  • Structural Biology
  • Phosphorylation and protein alosterism
  • Molecular microbiology
  • Signaling and regulation in bacteria
  • Adhikarla H, Wunder EA Jr, Mechaly AE, Mehta S, Wang Z, Santos L, Bisht V, Diggle P, Murray G, Adler B, Lopez F, Townsend JP, Groisman E, Picardeau M, Buschiazzo A, Ko AI. (2018) Lvr, a signaling system that controls global gene regulation and virulence in pathogenic Leptospira. Front Cell Infect Microbiol 8:45.
  • Wunder EA, Figueira CP, Benaroudj N, Hu B, Tong BA, Trajtenberg F, Liu J, Reis MG, Charon NW, Buschiazzo A, Picardeau M & Ko AI. (2016) A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Mol Microbiol. 101:457-70.
  • Trajtenberg F, Imelio JA, Machado MR, Larrieux N, Marti MA, Obal G, Mechaly AE, Buschiazzo A. (2016) Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action. eLife 5:e21422.
  • Fouts DE, Matthias MA, Adhikarla H, Adler B, Amorim-Santos L, Berg DE, Bulach D, Buschiazzo A, et al. (2016) What makes a bacterial species pathogenic?: comparative genomic analysis of the genus Leptospira. PLoS Negl Trop Dis. 10:e0004403.
  • Obal G, Trajtenberg F, Carrión F, Tomé L, Larrieux N, Zhang X, Pritsch O, Buschiazzo A. (2015) Conformational plasticity of a native retroviral capsid revealed by X-ray crystallography. Science 349:95-8.
  • Methot SP, Litzler LC, Trajtenberg F, Zahn A, Robert F, Pelletier J, Buschiazzo A, Magor BG, Di Noia JM. (2015) Consecutive interactions with HSP90 and eEF1A underlie a functional maturation and storage pathway of AID in the cytoplasm. J Exp Med. 212:581-96.
  • Trajtenberg F, Albanesi D, Ruétalo N, Botti H, Mechaly AE, Nieves M, Aguilar PS, Cybulski L, Larrieux N, de Mendoza D, Buschiazzo A. (2014) Allosteric activation of bacterial response regulators: the role of the cognate histidine kinase beyond phosphorylation. mBio. 5:e02105.
  • Morero NR, Botti H, Nitta KR, Carrión F, Obal G, Picardeau M, Buschiazzo A. (2014) HemR is an OmpR/PhoB-like response regulator from Leptospira, which simultaneously effects transcriptional activation and repression of key haem metabolism genes. Mol Microbiol. 94:340-52.
  • Trajtenberg F, Graña M, Ruétalo N, Botti H, Buschiazzo A. (2010) Structural and enzymatic insights into the ATP binding and autophosphorylation mechanism of a sensor histidine kinase. J Biol Chem. 285:24892-903.
  • Albanesi D, Martín M, Trajtenberg F, Mansilla MC, Haouz A, Alzari PM, de Mendoza D & Buschiazzo A. (2009) Structural plasticity and catalysis regulation of a thermosensor histidine kinase. Proc Natl Acad Sci USA. 106:16185-90.