Leonel Malacrida

Leonel Malacrida, PhD

Unit of
Advanced Bioimaging

  • BSc Biochemistry, Faculty of Sciences, University of the Republic, Uruguay (1999 – 2009).
  • PhD Biology, Biochemistry option. “Pulmonary Surfactant during Acute Lung Injury: From the pathophysiology to the biophysical aspects of its dysfunction”, University of the Republic, Uruguay (2009 – 2014).
  • PostDoc Biophysics/Biomedical Engineering, «Development of spectroscopy tools using lifetime and hyperspectral imaging for addressing biology quantitatively», Laboratory for Fluorescence Dynamics, University of California at Irvine, USA (2015-2019).
  • FLIM
  • Hyperspectral imaging
  • Phasor Plots
  • Fiber-optic based instruments
  • Fluorescent Probes
  • Biophysics of the cellular interior
  • Energy metabolism and autofluorescence
  • Phasor-based hyperspectral snapshot microscopy allows fast imaging of live, three-dimensional tissues for biomedical applications. Hedde PN, Cinco R, Malacrida L, Kamaid A, Gratton E.Commun Biol. 2021 Jun 11;4(1):721. doi: 10.1038/s42003-021-02266-z.
  • The Phasor Plot: A Universal Circle to Advance Fluorescence Lifetime Analysis and Interpretation. Malacrida L, Ranjit S, Jameson DM, Gratton E.Annu Rev Biophys. 2021 May 6;50:575-593. doi: 10.1146/annurev-biophys-062920-063631.
  • A global view of standards for open image data formats and repositories. Swedlow JR, Kankaanpää P, Sarkans U, Goscinski W, Galloway G, Malacrida L, Sullivan RP, Härtel S, Brown CM, Wood C, Keppler A, Paina F, Loos B, Zullino S, Longo DL, Aime S, Onami S.Nat Methods. 2021 May 4. doi: 10.1038/s41592-021-01113-7.
  • LAURDAN since Weber: The Quest for Visualizing Membrane Heterogeneity. Gunther G, Malacrida L, Jameson DM, Gratton E, Sánchez SA.Acc Chem Res. 2021 Feb 16;54(4):976-987. doi: 10.1021/acs.accounts.0c00687.
  • CAPRYDAA, an anthracene dye analog to LAURDAN: a comparative study using cuvette and microscopy. Castro-Castillo V, Gajardo J, Sandoval-Altamirano C, Gratton E, Sanchez S, Malacrida L, Gunther G.J Mater Chem B. 2020 Jan 7;8(1):88-99. doi: 10.1039/c9tb01738k.
  • The DIVER Microscope for Imaging in Scattering Media. Dvornikov A, Malacrida L, Gratton E.Methods Protoc. 2019 Jun 21;2(2):53. doi: 10.3390/mps2020053.
  • LAURDAN fluorescence and phasor plots reveal the effects of a H2O2 bolus in NIH-3T3 fibroblast membranes dynamics and hydration. Malacrida L, Gratton E.Free Radic Biol Med. 2018 Nov 20;128:144-156. doi: 10.1016/j.freeradbiomed.2018.06.004.
  • Fit-free analysis of fluorescence lifetime imaging data using the phasor approach. Ranjit S, Malacrida L, Jameson DM, Gratton E.Nat Protoc. 2018 Sep;13(9):1979-2004. doi: 10.1038/s41596-018-0026-5.
  • Comparison between iMSD and 2D-pCF analysis for molecular motion studies on in vivo cells: The case of the epidermal growth factor receptor. Malacrida L, Rao E, Gratton E.Methods. 2018 May 1;140-141:74-84. doi: 10.1016/j.ymeth.2018.01.010.
  • Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions. Malacrida L, Hedde PN, Ranjit S, Cardarelli F, Gratton E.Biomed Opt Express. 2017 Dec 20;9(1):303-321. doi: 10.1364/BOE.9.000303.
  • A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes. Malacrida L, Jameson DM, Gratton E.Sci Rep. 2017 Aug 23;7(1):9215. doi: 10.1038/s41598-017-08564-z.